CD33L3 (Active) protein (Biotin)
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北京 101111
Quick Overview for CD33L3 (Active) protein (Biotin) (ABIN7319803)
抗原
蛋白类型
生物活性
宿主
资源
纯度
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标记
- Biotin
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原理
- Recombinant Human Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-FC-Avi) Biotinylated
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序列
- Phe20-Thr263
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产品特性
- Biotinylated Recombinant Human Sialic Acid-binding Ig-like Lectin 15 is produced by our Mammalian expression system and the target gene encoding Phe20-Thr263 is expressed with a Fc, Avi tag at the C-terminus.
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内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
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Biological Activity Comment
- Immobilized Human Siglec-15-Fc-Avi (Cat#CY14) at 2μg/ml (100 μl/well) can bind Anti-Human Siglec15 mAb. The ED50 of Anti-Human Siglec15 mAb is 13.7 ng/ml.
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说明
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58-75 kDa
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from a 0.2 μm filtered solution of PBS, 150 mM NaCl, 0.3 % Chaps, 5 % Trehalose, pH 7.4.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- CD33L3
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背景
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Background: Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO--terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.
Synonym: Sialic acid-binding Ig-like lectin 15, Siglec-15, CD33 antigen-like 3, CD33L3
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分子量
- 55.3 kDa
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UniProt
- Q6ZMC9
抗原
-
