HCoV-NL63 Nucleocapsid protein (His tag)
This Recombinant protein is expressed in 大肠杆菌（E. Coli）.
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Quick Overview for HCoV-NL63 Nucleocapsid protein (His tag) (ABIN7198832)
抗原
HCoV-NL63 Nucleocapsid
蛋白类型
Recombinant
宿主
Human Coronavirus NL63 (HCoV-NL63)
资源
大肠杆菌（E. Coli）
纯度
> 95 % as determined by SDS-PAGE.
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标记
- His tag
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原理
- Recombinant Human coronavirus (HCoV-NL63) Nucleoprotein / NP Protein (His Tag)
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序列
- Met1-His377
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产品特性
- A DNA sequence encoding the Human coronavirus (HCoV-NL63) Nucleoprotein (YP_003771.1) (Met1-His377) was expressed with a polyhistide tag at the N-terminus.
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内毒素水平
- Please contact us for more information.
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HCoV-NL63 Nucleocapsid (AA 1-377) protein (His tag)
宿主: Human Coronavirus NL63 (HCoV-NL63) 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from sterile 40 mM PB 500 mM NaCl, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- HCoV-NL63 Nucleocapsid
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背景
- Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
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分子量
- 43.21kDa.
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NCBI登录号
- YP_003771
抗原
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