ZIKV E Protein (AA 291-399, AA 426-485) (His-MBP)
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北京 101111
Quick Overview for ZIKV E Protein (AA 291-399, AA 426-485) (His-MBP) (ABIN7198748)
抗原
蛋白类型
宿主
资源
纯度
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产品特性
- AA 291-399, AA 426-485
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标记
- This ZIKV E protein is labelled with His-MBP.
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原理
- Recombinant ZIKV E / Envelope protein (Domain I, His & MBP Tag)
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序列
- Ile291-Thr399 and Glu426-Gly485 and Ser576- Gly592
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产品特性
- A DNA sequence encoding the Zika virus (strain Zika SPH2015) E (ALU33341.1) (Ile291-Thr399 and Glu426-Gly485 and Ser576- Gly592) was expressed with a N-terminal polyhistidine-tagged MBP tag at the N-terminus (his-MBP).
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过滤
- 0.2 μm filtered
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Biological Activity Comment
- Not validated for activity
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Zika Virus Envelope (ZIKV E) (AA 593-699) protein (His tag)
ZIKV E 宿主: Zika Virus (ZIKV) 宿主: HEK-293 Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
Zika Virus Envelope (ZIKV E) (AA 593-699) protein (His tag)ZIKV E 宿主: Zika Virus (ZIKV) 宿主: 酵母菌 Recombinant > 95 % as determined by reducing SDS-PAGE.
Zika Virus Envelope (ZIKV E) (AA 593-699) protein (Fc Tag)ZIKV E 宿主: Zika Virus (ZIKV) 宿主: HEK-293 Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
Zika Virus Envelope (ZIKV E) (AA 291-694) protein (His tag)ZIKV E 宿主: Zika Virus (ZIKV) 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile 250 mM NaCl, 50 mM Tris, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- ZIKV E (Zika Virus Envelope (ZIKV E))
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别名
- Zika Envelope
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背景
- E protein,Zika virus,strain Zika SPH2015,Envelope of Zika virus is resposible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent to those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross reactivity with dengue and YF.Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76 % of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.
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分子量
- 57.2 kDa
抗原
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