VEGF-165 / VEGF-A (AA 27-191) (Active) 蛋白
This Recombinant VEGF-165 / VEGF-A protein is expressed in HEK-293 Cells.
产品编号 ABIN7319413
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Quick Overview for VEGF-165 / VEGF-A (AA 27-191) (Active) 蛋白 (ABIN7319413)
抗原
VEGF-165 / VEGF-A
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
HEK-293 Cells
应用范围
ELISA
纯度
> 95 % as determined by reducing SDS-PAGE.
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产品特性
- AA 27-191
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原理
- Recombinant Human VEGF165/VEGFA Protein
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序列
- Ala27-Arg191
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产品特性
- Recombinant Human VEGF165 is produced by our Mammalian expression system and the target gene encoding Ala27-Arg191 is expressed.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 0.01 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Immobilized Human VEGF165 (PKSH033475) at 10μg/ml (100 μl/well) can bind Human VEGFR1-Fc (PKSH033445). The ED50 of Recombinant Human VEGFR1-Fc (PKSH033445) is 36.4 ng/ml.
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from a 0.2 μm filtered solution of 20 mM Citrate, 8 % Sucrose, 4 % Mannitol, 0.05 % Tween 80, pH 4.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- VEGF-165 / VEGF-A
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别名
- VEGF165/VEGFA
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背景
- MVCD1,VEGF,VEGF165,VPF,Vascular Endothelial Growth Factor Isoform 165,Human Vascular endothelial growth factor (VEGF), also known as VEGF-A and vascular permeability factor (VPF), belongs to the platelet-derived growth factor family of cysteine-knot growth factors. It is a potent activator in vasculogenesis and angiogenesis both physiologically and pathologically. VEGF-A has 8 differently spliced isoforms, of which VEGF165 is the most abundant one. VEGF165 is a disulfide-linked homodimer consisting of two glycosylated 165 amino acid polypeptide chains. VEGF stimulates the cellular response through binding to tyrosine kinase receptors VEGFR1 and VEGFR2 on the cell surface. It is widely accepted that VEGFR2 mediate almost all of the known cellular responses to VEGF while the function of VEGFR1 is less defined and is thought to modulate the VEGFR2 signaling.
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分子量
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Calculated MW: 19.1 kDa
Observed MW: 18-24 kDa
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基因ID
- 7422
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UniProt
- P15692
抗原
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