UBE2I 蛋白
Quick Overview for UBE2I 蛋白 (ABIN7198588)
抗原
See all UBE2I 蛋白蛋白类型
宿主
资源
纯度
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原理
- Recombinant Human UBE2I/UBC9 Protein
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序列
- Met 1-Ser 158
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产品特性
- A DNA sequence encoding the human UBE2I (P63279) (Met 1-Ser 158) was expressed and purified, with additional two amino acids (Gly & Pro) at the N-terminus.
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Ubiquitin-Conjugating Enzyme E2I (UBE2I) (AA 1-158) protein (GST tag)
UBE2I 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2I (UBE2I) (AA 1-157), (partial) protein (GST tag)UBE2I 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
Ubiquitin-Conjugating Enzyme E2I (UBE2I) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)UBE2I 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ubiquitin-Conjugating Enzyme E2I (UBE2I) (Transcript Variant 4) protein (Myc-DYKDDDDK Tag)UBE2I 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ubiquitin-Conjugating Enzyme E2I (UBE2I) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)UBE2I 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ubiquitin-Conjugating Enzyme E2I (UBE2I) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)UBE2I 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from sterile PBS, 10 % glycerol, 2 mM DTT, pH 7.4
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))
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别名
- UBE2I/UBC9
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背景
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Background: UBE2I is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation; ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction; and thereby the fate of the substrate; is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space; a highly sophisticated conjugation machinery has been developed. In a sequential manner; ubiquitin becomes activated by an ubiquitin-activating enzyme (E1); which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next; ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system; wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans.
Synonym: SUMO-Conjugating Enzyme UBC9; SUMO-Protein Ligase; Ubiquitin Carrier Protein 9; Ubiquitin Carrier Protein I; Ubiquitin-Conjugating Enzyme E2 I; Ubiquitin-Protein Ligase I; p18; UBE2I; UBC9; UBCE9
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UniProt
- P63279
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway
抗原
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