Tyrosine Hydroxylase Protein (TH) (His tag)
Quick Overview for Tyrosine Hydroxylase Protein (TH) (His tag) (ABIN7320079)
抗原
See all Tyrosine Hydroxylase (TH) 蛋白蛋白类型
宿主
资源
纯度
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标记
- This Tyrosine Hydroxylase protein is labelled with His tag.
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原理
- Recombinant Mouse TH/Tyrosine Hydroxylase Protein (His Tag)
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序列
- Pro 2-Ser 498
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产品特性
- A DNA sequence encoding the mouse TH (P24529) (Pro 2-Ser 498) was fused with a polyhistidine tag at the N-terminus.
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Tyrosine Hydroxylase (TH) (Transcript Variant 2), (Tyr3) protein (Myc-DYKDDDDK Tag)
TH 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Tyrosine Hydroxylase (TH)
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别名
- TH/Tyrosine Hydroxylase
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背景
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Background: Tyrosine hydroxylase (TH) is a rate-limiting enzyme in catecholamine synthesis. Tyrosine hydroxylase activity is modulated by protein-protein interactions with enzymes in the same pathway or the tetrahydrobiopterin pathway, structural proteins considered to be chaperones that mediate the neuron's oxidative state. It is phosphorylated at serine (Ser) residues Ser8, Ser19, Ser31 and Ser40 in vitro. The phosphorylation of tyrosine hydroxylase at Ser19 or Ser8 has no direct effect on tyrosine hydroxylase activity. As tyrosine hydroxylase (TH) catalyses the formation of L-DOPA, the rate-limiting step in the biosynthesis of DA, the Parkinson's disease (PD) can be considered as a TH-deficiency syndrome of the striatum. A direct pathogenetic role of TH has also been suggested, as the enzyme is a source of reactive oxygen species (ROS) in vitro and a target for radical-mediated oxidative injury. Recently, it has been demonstrated that L-DOPA is effectively oxidized by mammalian Tyrosine hydroxylase in vitro, possibly contributing to the cytotoxic effects of DOPA.
Synonym: TH
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分子量
- 58 kDa
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UniProt
- P24529
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途径
- Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour
抗原
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