TLK2 Protein (His-GST)
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Quick Overview for TLK2 Protein (His-GST) (ABIN7317072)
抗原
See all TLK2 蛋白蛋白类型
宿主
资源
纯度
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标记
- This TLK2 protein is labelled with His-GST.
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原理
- Recombinant Human TLK2/PKU-ALPHA Protein (His&GST Tag)
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序列
- Leu 397-Asn 772
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产品特性
- A DNA sequence encoding the amino acids (Leu 397-Asn 772) of human TLK2 (Q86UE8-1) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Tousled-Like Kinase 2 (TLK2) protein
TLK2 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Tousled-Like Kinase 2 (TLK2) (Transcript Variant A) protein (Myc-DYKDDDDK Tag)TLK2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, 0.5 mM PMSF, pH 8.0
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储存液
- PMSF
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储存条件
- -20 °C
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储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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有效期
- 6 months
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- TLK2 (Tousled-Like Kinase 2 (TLK2))
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别名
- TLK2/PKU-ALPHA
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背景
- HsHPK,PKU-ALPHA,Serine / threonine-protein kinase tousled-like 2, also known as PKU-alpha, Tousled-like kinase 2 and TLK2, is a nucleus protein which belongs to theprotein kinase superfamily and Ser/Thr protein kinase family. The tousled-like kinases are an evolutionarily conserved family of proteins implicated in DNA repair, DNA replication and mitosis in metazoans and plants. Their absence from the yeasts and other eukaryotic 'microbes' suggests a specific role for them in the development of multicellular organisms. Tousled-like kinase 2 / TLK2 is widely expressed. It is present in fetal placenta, liver, kidney, pancreas, heart and skeletal muscle. It is also found in adult cell lines. Tousled-like kinase 2 / TLK2 contains oneprotein kinase domain. Tousled-like kinase 2 / TLK2 is rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent and appears to regulate processes involved in chromatin assembly.
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分子量
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Calculated MW: 71.3 kDa
Observed MW: 65 kDa
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基因ID
- 11011
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UniProt
- Q86UE8
抗原
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