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Quick Overview for CD43 Protein (Fc Tag) (ABIN7320163)
抗原
See all CD43 (SPN) 蛋白蛋白类型
宿主
资源
纯度
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标记
- This CD43 protein is labelled with Fc Tag.
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原理
- Recombinant Mouse SPN/CD43 Protein (Fc Tag)
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序列
- Met 1-Gly 248
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产品特性
- A DNA sequence encoding the extracellular domain of mouse SPN (P15702) (Met 1-Gly 248) was fused with the Fc region of human IgG1 at the C-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Sialophorin (SPN) (AA 20-253) protein (Fc Tag)
SPN 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Sialophorin (SPN) (AA 20-253) (Active) protein (His tag)SPN 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE Active
Sialophorin (SPN) (AA 20-248) (Active) protein (His tag)SPN 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Sialophorin (SPN) (AA 1-400) protein (GST tag)SPN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sialophorin (SPN) (AA 20-253) protein (His tag)SPN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % SDS
Sialophorin (SPN) (AA 27-253) protein (His tag)SPN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- CD43 (SPN) (Sialophorin (SPN))
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别名
- SPN/CD43
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背景
- A630014B01Rik,Cd43,Galgp,Ly-48,Ly48,CD43 is an abundantly expressed molecule on the T-cell surface that shows distinct localization to the migrating T-cell uropod and the distal pole complex (DPC) opposite the immunological synapse via association with the ezrin-radixin-moesin (ERM) family of actin regulatory proteins. CD43 has a 235-amino acid (aa) extracellular domain, a 23-aa transmembrane domain, and a 123-aa cytoplasmic domain, all encoded by a single exon. The intracytoplasmic region of the protein is necessary to transduce signals, it is rich in potentially phosphorylable threonines and serines but lacks tyrosine residues as well as catalytic activity. CD43 engagement on human peripheral blood T cells and monocytes leads to cell activation and proliferation through the generation of second messengers such as diacylglycerol and inositol phosphates, protein kinase C (PKC) activation and Ca2+ mobilization. In addition, CD43 ligation on human T cells induces the association of CD43 with Src family kinases, presumably through the interaction of their Src homology 3 domain with a proline-rich region of the CD43 intracytoplasmic tail. This molecule has been implicated in T cell activation, enhancing T cell response to allogeneic or mitogenic stimulation and CD43-specific signals have been reported to be sufficient to activate T cells in the absence of T cell receptor (TCR) engagement. In summary, CD43 regulates multiple T-cell functions, including T-cell activation, proliferation, apoptosis, and migration.
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分子量
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Calculated MW: 49.6 kDa
Observed MW: 110 kDa
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基因ID
- 20737
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UniProt
- P15702
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途径
- Regulation of Leukocyte Mediated Immunity
抗原
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