PKD2 Protein (His-GST)
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Quick Overview for PKD2 Protein (His-GST) (ABIN7317066)
抗原
See all PKD2 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This PKD2 protein is labelled with His-GST.
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原理
- Recombinant Human PRKD2/PKD2 Protein (His & GST Tag)
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序列
- Met 1-Leu 878
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产品特性
- A DNA sequence encoding the amino acid sequence (Met 1-Leu 878) of human PRKD2 (NP_057541.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- The specific activity was determined to be > 30 nmol/min/mg using synthetic CREBtide peptide (KRREILSRRPSYR) as substrate.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Supplied as sterile solution of 50 mM Tris, 500 mM NaCl, 0.5 mM PMSF, 10 % glycerol, pH 8.0
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储存液
- PMSF
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储存条件
- -20 °C
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储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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有效期
- 6 months
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- PKD2 (Protein Kinase D2 (PKD2))
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别名
- PRKD2/PKD2
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背景
- HSPC187,PKD2,nPKC-D2,Serine/threonine-protein kinase D2, also known as PRKD2 and PKD2, is a cytoplasm and membrane protein which belongs to theprotein kinase superfamily, CAMK Ser/Thr protein kinase family and PKD subfamily. PRKD2 / PKD2 is widely expressed. It contains onePH domain, twophorbol-ester/DAG-type zinc fingers and oneprotein kinase domain. PRKD2 / PKD2 is activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains bind DAG, mediating translocation to membranes. Autophosphorylation of Ser-710 and phosphorylation of Ser-706 by PKC relieves auto-inhibition by the PH domain. PRKD2 / PKD2 converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
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分子量
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Calculated MW: 124 kDa
Observed MW: 120 kDa
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基因ID
- 25865
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NCBI登录号
- NP_057541
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UniProt
- Q9BZL6
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途径
- cAMP Metabolic Process, Maintenance of Protein Location, Negative Regulation of Transporter Activity
抗原
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