PREP Protein (His tag)
Quick Overview for PREP Protein (His tag) (ABIN7197520)
抗原
See all PREP 蛋白蛋白类型
宿主
资源
纯度
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标记
- This PREP protein is labelled with His tag.
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原理
- Recombinant Mouse Prolyl endopeptidase/PREP Protein (His Tag)
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序列
- Leu2-Gln710
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产品特性
- A DNA sequence encoding the mature form of mouse PREP (Q9QUR6) (Leu2-Gln710) was expressed, with a polyhistidine tag at the N-terminus.
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Prolyl Endopeptidase (PREP) (AA 1-710) protein (His tag)
custom-made PREP 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Prolyl Endopeptidase (PREP) (AA 1-710) protein (His tag)custom-made PREP 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Prolyl Endopeptidase (PREP) (AA 611-710) protein (GST tag)PREP 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Prolyl Endopeptidase (PREP) (AA 1-710) protein (GST tag)PREP 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Prolyl Endopeptidase (PREP) (AA 1-710) protein (His tag)PREP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90% by SDS-PAGE SDS
Prolyl Endopeptidase (PREP) protein (Myc-DYKDDDDK Tag)PREP 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol, 3 mM DTT
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- PREP (Prolyl Endopeptidase (PREP))
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别名
- Prolyl endopeptidase/PREP
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背景
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Background: Prolyl endopeptidase, also known as PREP, belongs to a distinct class of serine peptidases. It is a large cytosolic enzyme which was first described in the cytosol of rabbit brain as an oligopeptidase. Prolyl endopeptidase degrades the nonapeptide bradykinin at the Pro-Phe bond. It is involved in the maturation and degradation of peptide hormones and neuropeptides such as alpha-melanocyte-stimulating hormone, luteinizing hormone-releasing hormone (LH-RH), thyrotropin-releasing hormone, angiotensin, neurotensin, oxytocin, substance P and vasopressin. Prolyl endopeptidase's activity is confined to action on oligopeptides of less than 10 kD and it has an absolute requirement for the trans-configuration of the peptide bond preceding proline. It cleaves peptide bonds at the C-terminal side of proline residues.
Synonym: AI047692,AI450383,D10Wsu136e,PEP,Pop
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分子量
- 82.9 kDa
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UniProt
- Q9QUR6
抗原
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