Podoplanin Protein (PDPN) (His-Fc tag)
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Quick Overview for Podoplanin Protein (PDPN) (His-Fc tag) (ABIN7320346)
抗原
See all Podoplanin (PDPN) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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标记
- This Podoplanin protein is labelled with His-Fc tag.
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原理
- Recombinant Mouse Podoplanin/PDPN Protein (His & Fc Tag)
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序列
- Met 1-Leu 141
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产品特性
- A DNA sequence encoding the extracellular domain (Met 1-Leu 141) of mouse PDPN (NP_034459.2) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Immobilized mouse PDPN-Fch at 10 μg/ml (100 μl/well) can bind biotinylated human CLEC1B-His, The EC50 of biotinylated human CLEC1B-His is 0.04-0.08 μg/ml.
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Podoplanin (PDPN) (AA 1-238) protein (GST tag)
PDPN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Podoplanin (PDPN) (AA 23-141) protein (His-IF2DI Tag)PDPN 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Podoplanin (PDPN) (AA 99-207) protein (His tag)PDPN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS
Podoplanin (PDPN) (AA 23-133) protein (Fc Tag)PDPN 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
Podoplanin (PDPN) (AA 1-135), (Extracellular Domain) protein (Fc Tag)PDPN 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Podoplanin (PDPN) protein (His tag)PDPN 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 92 % as determined by reducing SDS-PAGE.
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- Podoplanin (PDPN)
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别名
- Podoplanin/PDPN
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背景
- Aggrus,Glycoprotein 38,Gp38,OTS-8,PA2.26 antigen,Podoplanin,RANDAM-2,T1-alpha,T1a,T1alpha,Podoplanin, also known asPDPN, is a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined. Alternatively spliced transcript variants encoding different isoforms have been identified.PDPN is a mucin-type glycoprotein negatively charged by extensive O-glycosylation and a high content of sialic acid, which expresses the adhesive property. It is selectively expressed in lymphatic endothelium as well as lymphangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. PDPN may contribute to form odontoblastic fiber or function as the anchorage to the tooth development and in proliferating epithelial cells of cervical loop and apical bud. The intensity of podoplanin expression is negatively correlated with the expression of CD34 and factor VIII. Podoplanin would be useful as a diagnostic marker for epithelioid hemangioendothelioma in liver tumors.
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分子量
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Calculated MW: 40.6 kDa
Observed MW: 60-65 kDa
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基因ID
- 14726
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NCBI登录号
- NP_034459
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UniProt
- Q62011
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途径
- Dicarboxylic Acid Transport
抗原
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