PDGF-BB Homodimer (Active) protein (His tag)
Quick Overview for PDGF-BB Homodimer (Active) protein (His tag) (ABIN7320790)
抗原
蛋白类型
生物活性
宿主
资源
纯度
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标记
- His tag
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原理
- Recombinant Mouse PDGF-BB Protein (His Tag)(Active)
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序列
- Ser82-Thr190
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产品特性
- Recombinant Mouse PDGF-BB is produced by our E.coli expression system and the target gene encoding Ser82-Thr190 is expressed with a 6His tag at the C-terminus.
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内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
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Biological Activity Comment
- Measured in a cell proliferation assay using Balb/c3T3 mouse fibroblast cells. The ED50 for this effect is 1.55 ng/ml.
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PDGF-BB Homodimer (Active) protein (His tag)
宿主: 人 宿主: 酵母菌 Recombinant > 95 % as determined by reducing SDS-PAGE. Active
PDGF-BB Homodimer protein宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 98 % as determined by reducing SDS-PAGE.
PDGF-BB Homodimer (AA 82-190) (Active) protein宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >90 % as determined by reducing (R) and non-reducing (NR) SDS-PAGE. Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from a 0.2 μm filtered solution of 4 mM HCl.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- PDGF-BB Homodimer
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别名
- PDGF-BB
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背景
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Background: Platelet-Derived Growth Factor Subunit B (PDGFB) belongs to the PDGF/VEGF growth factor family. Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. As growth factor,it plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. It is required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. PDGFB also plays an important role in wound healing.
Synonym: Platelet-Derived Growth Factor Subunit B, PDGF Subunit B, PDGF-2, Platelet-Derived Growth Factor B Chain, Platelet-Derived Growth Factor Beta Polypeptide, Proto-Oncogene c-Sis, Becaplermin, PDGFB, PDGF2, SIS
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分子量
- 13.4 kDa
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UniProt
- P31240
抗原
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