PFKM Protein (His-GST)
This Recombinant PFKM protein is expressed in Baculovirus infected Insect Cells.
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产品编号 ABIN7197337
发货至:
中国
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北京 101111
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Quick Overview for PFKM Protein (His-GST) (ABIN7197337)
抗原
See all PFKM 蛋白
PFKM
(phosphofructokinase, Muscle (PFKM))
蛋白类型
Recombinant
宿主
人
资源
Baculovirus infected Insect Cells
纯度
> 90 % as determined by reducing SDS-PAGE.
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标记
- This PFKM protein is labelled with His-GST.
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原理
- Recombinant Human PFK1/PFKM Protein (His & GST Tag)
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序列
- Thr 2-Val 780
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产品特性
- A DNA sequence encoding the human PFKM (P08237-1) (Thr2-Val780) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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phosphofructokinase, Muscle (PFKM) protein (GST tag)
PFKM 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
phosphofructokinase, Muscle (PFKM) protein (Myc-DYKDDDDK Tag)PFKM 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.5, 10 % glycerol
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储存条件
- -20 °C
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储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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有效期
- 6 months
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- PFKM (phosphofructokinase, Muscle (PFKM))
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别名
- PFK1/PFKM
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背景
- 6-phosphofructokinase,ATP-PFK,GSD7,PFK-1,PFK1,PFKA,PFKM,PFKX,PPP1R122,Phosphofructo-1-kinase isozyme A,Phosphofructokinase 1,Phosphohexokinase,muscle type,PFK1, also known as PFKM, is a regulatory glycolytic enzyme. PFK1 converts fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. It is a muscle-type isozyme. There are three phosphofructokinase isozymes in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Mutations in PFK1 gene have been related with glycogen storage disease type VII, also identified as Tarui disease.
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分子量
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Calculated MW: 112.9 kDa
Observed MW: 113 kDa
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基因ID
- 5213
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UniProt
- P08237
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途径
- Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effect
抗原
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