NEK3 Protein (His-GST)
This Recombinant NEK3 protein is expressed in Baculovirus infected Insect Cells.
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产品编号 ABIN7320002
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Quick Overview for NEK3 Protein (His-GST) (ABIN7320002)
抗原
See all NEK3 蛋白
NEK3
(NIMA related kinase 3 (NEK3))
蛋白类型
Recombinant
生物活性
Active
宿主
小鼠
资源
Baculovirus infected Insect Cells
纯度
> 90 % as determined by reducing SDS-PAGE.
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标记
- This NEK3 protein is labelled with His-GST.
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原理
- Recombinant Mouse NEK3 Protein (His & GST Tag)
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序列
- Met 1-Ala 509
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产品特性
- A DNA sequence encoding the mouse NEK3 (ABK42288.1) (Met 1-Ala 509) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- The specific activity was determined to be 8 nmol/min/mg using MBP as substrate.
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NIMA related kinase 3 (NEK3) (AA 1-506) protein (GST tag)
NEK3 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol
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储存条件
- -20 °C
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储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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有效期
- 6 months
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- NEK3 (NIMA related kinase 3 (NEK3))
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别名
- NEK3
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背景
- NEK3,NEK3 (NIMA (never in mitosis gene a)-related expressed kinase 3), contains 1 protein kinase domain and is a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. Members of the NEK family of protein kinases share high amino acid homology with NIMA (never in mitosis gene a). NEK3 differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. It is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. NEK3 mRNA can be detected in all the proliferating cell lines with the amount not changing during the cell cycle. Prolactin stimulates interaction between NEK3 and paxillin leading to increased paxillin phosphorylation, Analysis of breast tissue microarrays show a significant up-regulation of NEK3 expression in malignant versus normal specimens. Multiple transcript variants encoding different isoforms have been found for NEK3 gene. NEK3 may play a role in mitotic regulation.
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分子量
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Calculated MW: 84.8 kDa
Observed MW: 72 kDa
抗原
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