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NAMPT Protein (His-GST)

This Recombinant NAMPT protein is expressed in Baculovirus infected Insect Cells.
产品编号 ABIN7318084
发货至: 中国
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Quick Overview for NAMPT Protein (His-GST) (ABIN7318084)

抗原

See all NAMPT 蛋白
NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

蛋白类型

Recombinant

宿主

  • 16
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资源

  • 18
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Baculovirus infected Insect Cells

纯度

> 90 % as determined by reducing SDS-PAGE.
  • 标记

    This NAMPT protein is labelled with His-GST.

    原理

    Recombinant Human PBEF/NAMPT Protein (His & GST Tag)

    序列

    Met 1-His 491

    产品特性

    A DNA sequence encoding the human NAMPT (P43490) (Met 1-His 491 was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

    过滤

    0.2 μm filtered

    内毒素水平

    < 1.0 EU per μg of the protein as determined by the LAL method.

    Biological Activity Comment

    Not validated for activity
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    Product
    表达系统
    Conjugate
    Origin
    价格从
    表达系统 HEK-293 Cells
    Conjugate His tag
    Origin Human
    价格从 116,946.14 ¥
    表达系统 Cell-free protein synthesis (CFPS)
    Conjugate Strep Tag
    Origin Human
    价格从 145,985.55 ¥

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  • 限制

    仅限研究用
  • 状态

    Lyophilized

    缓冲液

    Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 20 % glycerol, 0.3 mM DTT.
    Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

    储存液

    Dithiothreitol (DTT)

    注意事项

    This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

    有效期

    12 months
  • 抗原

    NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

    别名

    PBEF/NAMPT

    背景

    NAMPT,NAmPRTase,Nampt,Nicotinamide phosphoribosyltransferase,PBEF,PBEF1,Pre-B cell-enhancing factor,Pre-B-cell colony-enhancing factor 1,Visfatin,Nicotinamide phosphoribosyltransferase (NAMPT), also known as pre-B-cell colony-enhancing factor 1 (PBEF1) or visfatin, is an enzyme belonging to the family of glycosyltransferases, to be specific, the pentosyltransferases. This enzyme participates in nicotinate and nicotinamide metabolism. This enzyme catalyzes the condensation of nicotinamide with 5- phosphoribosyl-1- pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. NAMPT is also considered as an essential enzyme mediating granulocyte colony-stimulating factor (G-CSF)-triggered granulopoiesis in healthy individuals and in individuals with severe congenital neutropenia. Intracellular NAMPT and NAD+ amounts in myeloid cells, as well as plasma NAMPT and NAD+ levels, were increased by G-CSF treatment of both healthy volunteers and individuals with congenital neutropenia.

    分子量

    Calculated MW: 83.3 kDa

    Observed MW: 75 kDa

    基因ID

    10135

    UniProt

    P43490
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