NEK7 Protein (NEK7) (His-GST)
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Quick Overview for NEK7 Protein (NEK7) (His-GST) (ABIN7317545)
抗原
See all NEK7 蛋白蛋白类型
宿主
资源
纯度
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标记
- This NEK7 protein is labelled with His-GST.
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原理
- Recombinant Human NEK7 Protein (His & GST Tag)
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序列
- Met 1-Ser 302
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产品特性
- A DNA sequence encoding the human NEK7 (NP_598001.1) (Met1-Ser 302) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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NEK7 (NEK7) (AA 1-302) protein (His tag)
custom-made NEK7 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
NEK7 (NEK7) (AA 1-302) protein (GST tag)NEK7 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
NEK7 (NEK7) (AA 1-302) protein (His tag)NEK7 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.5, 0.5 mM Reduced Glutathione, 0.5 mM PMSF
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存液
- PMSF
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- NEK7
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别名
- NEK7
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背景
- NEK7,NIMA (never in mitosis gene a)-related kinase 7, NEK7 belongs to the NIMA subfamily, NEK Ser/Thr protein kinase family, protein kinase superfamily. NEKs (NIMA-related kinases) are mammalian serine/threonine (Ser/Thr) protein kinases structurally related to Aspergillus NIMA (Never in Mitosis, gene A), which plays essential roles in mitotic signaling. NEKs share an amino-terminal catalytic domain related to NIMA, an Aspergillus kinase involved in the control of several aspects of mitosis, and divergent carboxyl-terminal tails of varying length. NEKs are commonly referred to as mitotic kinases, although a definitive in vivo verification of this definition is largely missing. Reduction in the activity of NEK7 or its close paralog, NEK6, has previously been shown to arrest cells in mitosis, mainly at metaphase. NEK7 is a regulator of cell division, and reveal it as an essential component for mammalian growth and survival. The intimate connection between tetraploidy, aneuploidy and cancer development suggests that NEK7 deregulation can induce oncogenesis. The endogenous NEK7 protein is enriched at the centrosome in a microtubule-independent manner. Overexpression of wt or kinase-defective NEK7 resulted in cells of rounder appearance, and higher proportions of multinuclear and apoptotic cells.
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分子量
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Calculated MW: 62.4 kDa
Observed MW: 58 kDa
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NCBI登录号
- NP_598001
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UniProt
- B2R8K8
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途径
- Inflammasome
抗原
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