TIMP2 蛋白
Quick Overview for TIMP2 蛋白 (ABIN7317884)
抗原
See all TIMP2 蛋白蛋白类型
生物活性
宿主
资源
纯度
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原理
- Recombinant Human TIMP2/TIMP-2 Protein (Active)
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序列
- Cys 27-Pro 220
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产品特性
- A DNA sequence encoding the mature form of human TIMP2 (NP_003246.1) (Cys 27-Pro 220) was expressed and purified.
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内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
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Biological Activity Comment
- Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate MCA-PLGL-DPA-AR-NH2(R&D Systems, Catalog # ES001). The IC50 value is < 4 nM.
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Metalloproteinase Inhibitor 2 (TIMP2) (AA 27-220) protein (His tag)
TIMP2 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Metalloproteinase Inhibitor 2 (TIMP2) (AA 27-220) protein (His tag)TIMP2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Metalloproteinase Inhibitor 2 (TIMP2) protein (Fc Tag)TIMP2 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Metalloproteinase Inhibitor 2 (TIMP2) (AA 27-220) protein (GST tag)TIMP2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Metalloproteinase Inhibitor 2 (TIMP2) (AA 30-220), (partial) protein (GST tag)TIMP2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from sterile PBS, pH 7.4
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))
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别名
- TIMP2/TIMP-2
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背景
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Background: Tissue inhibitors of metalloproteinases (TIMP) family are natural inhibitors of the matrix metalloproteinases (MMPs), the zinc enzymes involved in extracellular matrix maintenance and remodeling. The TIMP family encompasses four members (TIMP1-4), and they inhibit most MMPs by forming non-covalent binary complex. TIMP2 is a 22 kDa non N-glycosylated protein expressed by a variety of cell types, and plays a unique role among TIMP family members owing to its functions to regulate cellular responses to growth factors. Findings establish an unexpected, MMP-independent mechanism for TIMP2 inhibition of endothelial cell proliferation in vitro and reveal an important component of the antiangiogenic effect of TIMP2 in vivo. TIMP-2 thus is critical to the maintenance of tissue homeostasis and is involved in the regulation of tumor microenvironment.
Synonym: Metalloproteinase Inhibitor 2, CSC-21K, Tissue Inhibitor of Metalloproteinases 2,CSC-21K,DDC8
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分子量
- 22 kDa
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NCBI登录号
- NP_003246
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途径
- cAMP Metabolic Process
抗原
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