MMP2 蛋白
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北京 101111
Quick Overview for MMP2 蛋白 (ABIN7318047)
抗原
See all MMP2 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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原理
- Recombinant Human MMP-2 Protein
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序列
- Met 1-Cys 660
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产品特性
- A DNA sequence encoding the native human MMP2 (NP_004521.1) (Met 1-Cys 660) was expressed and purified.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- 1. Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 (AnaSpec, Catalog # 27076). The specific activity is > 1, 000 pmoles/min/μg. 2. Immobilized human MMP2 at 10 μg/mL (100 μl/well) can bind human TIMP2/Fc. The EC50 of human TIMP2/Fc is 0.02 μg/mL. (Activation description: The proenzyme needs to be activated by APMA for an activated form)
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Matrix Metalloproteinase 2 (MMP2) protein
MMP2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func
Matrix Metalloproteinase 2 (MMP2) (AA 30-660) protein (His tag)MMP2 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
Matrix Metalloproteinase 2 (MMP2) (AA 424-660) protein (His-IF2DI Tag)MMP2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Matrix Metalloproteinase 2 (MMP2) (AA 109-660) protein (His-IF2DI Tag)MMP2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Matrix Metalloproteinase 2 (MMP2) (AA 1-660) protein (GST tag)MMP2 宿主: 人 宿主: 小麦胚 Recombinant AP, AA, ELISA, WB
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- MMP2 (Matrix Metalloproteinase 2 (MMP2))
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别名
- MMP-2
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背景
- 72 kDa Gelatinase,72 kDa Type IV Collagenase,CLG4,CLG4A,Gelatinase A,MMP-2,MMP-II,MMP2,MONA,Matrix Metalloproteinase-2,TBE-1,Matrix Metalloproteinase-2 (MMP-2) is an enzyme that degrades components of the extracellular matrix and thus plays a pivotal role in cell migration during physiological and pathological processes. MMP-2 expression is dependent on extracellular matrix metalloproteinase inducer (EMMPRIN), Her2/neu, growth factors, cytokines, and hormones. Pro-MMP-2 activation needs MT1-MMP and TIMP-2 contribution. MMP-2 is changed in distribution and increased in amount in the ventral cochlear nucleus after unilateral cochlear ablation. A low level of MMP-2 is linked to favorable prognosis in patients with a hormone receptor-negative tumor, usually associated with high risk. As a zymogen requiring proteolytic activation for catalytic activity, MMP-2 has been implicated broadly in the invasion and metastasis of many cancer model systems, including human breast cancer (HBC). Blocking MMP-2 secretion and activation during breast carcinoma development may decrease metastasis. The detection of active MMP-2 alone or the rate of pro-MMP-2 and active MMP-2 is considered a very sensitive indicator of cancer metastasis. Modulation of MMP-2 expression and activation through specific inhibitors and activators may thus provide a new mechanism for breast cancer treatment.
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分子量
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Calculated MW: 72 kDa
Observed MW: 72 kDa
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基因ID
- 4313
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NCBI登录号
- NP_004521
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UniProt
- P08253
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途径
- Activation of Innate immune Response
抗原
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