KARS Protein (His tag)
Quick Overview for KARS Protein (His tag) (ABIN7318750)
抗原
See all KARS 蛋白蛋白类型
宿主
资源
纯度
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标记
- This KARS protein is labelled with His tag.
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原理
- Recombinant Human KARS Protein (His Tag)
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序列
- Ala2-Val597
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产品特性
- Recombinant Human Lysine--tRNA Ligase is produced by our Mammalian expression system and the target gene encoding Ala2-Val597 is expressed with a 6His tag at the C-terminus.
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内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
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Lysyl-tRNA Synthetase (KARS) (AA 1-597) protein (GST tag)
KARS 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Lysyl-tRNA Synthetase (KARS) (AA 63-597) protein (His tag)KARS 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Lysyl-tRNA Synthetase (KARS) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)KARS 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Frozen, Liquid
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缓冲液
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl,1 mM DTT,20 % glycerol, pH 8.0.
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储存条件
- -20 °C
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储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- KARS (Lysyl-tRNA Synthetase (KARS))
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别名
- KARS
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背景
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Background: Lysine-tRNA ligase, also known as Lysyl-tRNA synthetase, LysRS, KARS and KIAA0070, belongs to the class-II aminoacyl-tRNA synthetase family. The N-terminal cytoplasmic domain (1-65) is a functional tRNA-binding domain, which is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. KARS catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Defects in KARS are the cause of Charcot-Marie-Tooth disease recessive intermediate type B (CMTRIB).
Synonym: Lysine--tRNA Ligase, Lysyl-tRNA Synthetase, LysRS, KARS, KIAA0070
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分子量
- 69.1 kDa
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UniProt
- Q15046
抗原
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