ITCH 蛋白
Quick Overview for ITCH 蛋白 (ABIN7317650)
抗原
See all ITCH 蛋白蛋白类型
宿主
资源
纯度
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原理
- Recombinant Human ITCH/AIP4 Protein (aa 526-903)
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序列
- Arg 526-Glu 903
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产品特性
- A DNA sequence encoding the human ITCH (NP_113671.3) N-terminal segment (Arg 526-Glu 903) was expressed and purified, with two additional amino acids (Gly & Pro) at the N-terminus.
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Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 197-542) protein (His-IF2DI Tag)
ITCH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 92-190) protein (GST tag)ITCH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from sterile 20 mM Tris, 200 mM NaCl, 10 % glycerol, pH 8.0
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))
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别名
- ITCH/AIP4
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背景
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Background: E3 ubiquitin-protein ligase Itchy homolog, also known as Atrophin-1-interacting protein 4, NFE2-associated polypeptide 1, NAPP1 and ITCH, is a cell membrane protein which contains one C2 domain, one HECT (E6AP-type E3 ubiquitin-protein ligase) domain and contains four WW domains. ITCH acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation. ITCH is involved in the control of inflammatory signaling pathways. It is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. ITCH promotes the association of the complex after TNF stimulation. Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteosomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1. Defects in ITCH are the cause of syndromic multisystem autoimmune disease (SMAD) which is characterized by organomegaly, failure to thrive, developmental delay, dysmorphic features and autoimmune inflammatory cell infiltration of the lungs, liver and gut.
Synonym: ADMFD;AIF4;AIP4;dJ468O1.1;NAPP1
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分子量
- 40 kDa
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NCBI登录号
- NP_113671
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途径
- Activation of Innate immune Response, CXCR4-mediated Signaling Events
抗原
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