IL-4 Protein (AA 25-153)
Our Local Distributor
北京 101111
Quick Overview for IL-4 Protein (AA 25-153) (ABIN7319404)
抗原
See all IL-4 (IL4) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
-
-
产品特性
- AA 25-153
-
原理
- Recombinant Human Interleukin-4/IL-4 Protein
-
序列
- His25-Ser153
-
产品特性
- Recombinant Human Interleukin-4 is produced by our Mammalian expression system and the target gene encoding His25-Ser153 is expressed.
-
过滤
- 0.2 μm filtered
-
内毒素水平
- < 0.01 EU per μg of the protein as determined by the LAL method.
-
Biological Activity Comment
- Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.01-0.05 ng/ml.
-
-
Interleukin 4 (IL4) (AA 25-153) (Active) protein (Biotin,His-Avi Tag)
IL4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Interleukin 4 (IL4) (AA 25-153) (Active) protein (His tag)IL4 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Interleukin 4 (IL4) (AA 25-153) (Active) protein (His-Avi Tag)IL4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Interleukin 4 (IL4) (AA 25-153) (Active) proteinIL4 宿主: 人 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 4 (IL4) (AA 23-140) (Active) proteinIL4 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 4 (IL4) (AA 25-153) (Active) protein (His tag,AVI tag,Biotin)IL4 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 4 (IL4) (AA 63-153) protein (GST tag)IL4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Interleukin 4 (IL4) (AA 1-153) protein (GST tag)IL4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Interleukin 4 (IL4) (AA 25-153) protein (His tag)IL4 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Interleukin 4 (IL4) (AA 25-153) (Active) protein (His tag)IL4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
缓冲液
-
Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
有效期
- 12 months
-
-
- IL-4 (IL4) (Interleukin 4 (IL4))
-
别名
- Interleukin-4/IL-4
-
背景
- Interleukin,B-cell growth factor,BSF,IL4,BCGF-1,BCGF1,BSF-1,BSF1,IL-4,B-cell growth factor 1,B-cell IgG differentiation factor,B-cell stimulatory factor 1,Binetrakin,IGG1 induction factor,Interleukin-4,Lymphocyte stimulatory factor 1,Pitrakinra,Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response.
-
分子量
-
Calculated MW: 15.0 kDa
Observed MW: 18 kDa
-
基因ID
- 3565
-
UniProt
- P05112
-
途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
抗原
-
