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IgG1 Fc protein (Fc Tag)

宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE
产品编号 ABIN7196158
发货至: 中国
  • 抗原
    IgG1 Fc
    蛋白类型
    Recombinant
    宿主
    • 6
    • 2
    小鼠
    资源
    • 6
    • 1
    • 1
    HEK-293 Cells
    标记
    Fc Tag
    原理
    Recombinant Mouse IgG1-Fc Protein (102 Cys/Ser)
    序列
    Val 98-Lys 324
    产品特性
    The Fc region of mouse IgG1 (P01868-1) (Val 98-Lys 324) (one aa mutation, 102 Cys / Ser) was expressed and purified.
    纯度
    > 97 % as determined by SDS-PAGE
    内毒素水平
    < 1.0 EU per μg of the protein as determined by the LAL method.
  • 限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Please refer to the printed manual for detailed information.
    缓冲液
    Lyophilized from sterile 100 mM NaAc, 10 mM NaCl, 200 mM Tris, pH 7.5
    储存条件
    4 °C,-20 °C,-80 °C
    储存方法
    Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
  • 抗原
    IgG1 Fc
    别名
    IgG1-Fc
    背景

    Background: As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75 % of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues. Protein G or Protein A on the surface of certain Staphylococcal and Streptococcal strains specifically binds with the Fc region of IgGs, and has numerous applications in biotechnology as a reagent for affinity purification. Recombinant IgG Fc Region is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases.

    Synonym: IgG1,Igh-4,VH7183

    分子量
    25.8 kDa
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