IgG1 Fc (Active) protein (Fc Tag)
Quick Overview for IgG1 Fc (Active) protein (Fc Tag) (ABIN7318594)
抗原
蛋白类型
生物活性
宿主
资源
纯度
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标记
- Fc Tag
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原理
- Recombinant Human IgG1 Fc Protein (Active)
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序列
- Asp104-Lys330
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产品特性
- Recombinant Human Immunoglobulin G1 Fc is produced by our Mammalian expression system and the target gene encoding Asp104-Lys330 is expressed.
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内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
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Biological Activity Comment
- Immobilized Human IgG1 Fc at 2μg/ml(100 μl/well) can bind Human CD64-His(Cat: PKSH033655). The ED50 of Human IgG1 Fc is 47.99 ng/ml .
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IgG1 Fc (AA 104-330) protein
宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IgG1 Fc (AA 98-324) protein宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IgG1 Fc protein (Fc Tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE
IgG1 Fc protein宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. WB
IgG1 Fc (AA 100-330) protein (DYKDDDDK Tag)宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- IgG1 Fc
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背景
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Background: As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75 % of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity.
Synonym: g gamma-1 chain C region,IGHG1
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分子量
- 41.6 kDa
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UniProt
- P01857
抗原
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