Hemopexin Protein (HPX) (His tag)
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Quick Overview for Hemopexin Protein (HPX) (His tag) (ABIN7317735)
抗原
See all Hemopexin (HPX) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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标记
- This Hemopexin protein is labelled with His tag.
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原理
- Recombinant Human Hemopexin/HPX Protein (His Tag)
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序列
- Met 1-His 462
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产品特性
- A DNA sequence encoding the human HPX (NP_000604.1) (Met 1-His 462) with a C-terminal polyhistidine tag was expressed.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Measured by its ability to bind protoporphyrin IX (PPPIX). Recombinant human Hemopexin binds > 10 μM PPPIX, resulting in a 50% decrease in the fluorescence signal of human Hemopexin.
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Hemopexin (HPX) (AA 24-462) (Active) protein (His tag)
HPX 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Hemopexin (HPX) (AA 24-462) (Active) protein (His tag)HPX 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Hemopexin (HPX) (AA 24-460) (Active) protein (His tag)HPX 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Hemopexin (HPX) (AA 24-462) protein (His tag)HPX 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % SDS
Hemopexin (HPX) (AA 24-460) protein (His-IF2DI Tag)HPX 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Hemopexin (HPX) (Active) protein (His tag)HPX 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 98 % as determined by reducing SDS-PAGE. Active
Hemopexin (HPX) (AA 1-254) protein (GST tag)HPX 宿主: 人 宿主: 小麦胚 Recombinant AP, AA, ELISA, WB
Hemopexin (HPX) (AA 24-460) protein (His tag)HPX 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Hemopexin (HPX) (AA 24-460), (full length) protein (His tag)HPX 宿主: 大鼠 宿主: 酵母菌 Recombinant > 90 % ELISA
Hemopexin (HPX) proteinHPX 宿主: 人 宿主: Human Native >95 %
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- Hemopexin (HPX)
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别名
- Hemopexin/HPX
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背景
- Hemopexin,Hpx,Hpxn,Hemopexin (HPX) is plasma glycoprotein belongs to the family of the acute-phase proteins whose synthesis is induced after an inflammatory event. Hemopexin with two four-bladed beta -propeller folds has been found in other proteins including collagenases and provides sites for protein-protein interactions. The liver is the major synthesizing organ. Hemopexin participates in maintaining and recycling the iron pool by utilizing its high binding affinity toward heme composed of protoporphyrin IX and iron. It also functions in preventing oxidation caused by heme after hemolysis. Hydrophobic heme molecules can intercalate into lipid membranes and participate in the oxidation of lipid membrane components through the Fenton reaction resulting in lipid peroxidation. Hemopexin undergoes a conformational change upon the binding of heme. The conformational change allows hemopexin to interact with a specific receptor, forming a complex which is then internalized. Heme concentrations in plasma increase after hemolysis, which is associated with several pathological conditions such as reperfusion injury and ischemia.
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分子量
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Calculated MW: 50.7 kDa
Observed MW: 70-75 kDa
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基因ID
- 3263
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NCBI登录号
- NP_000604
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UniProt
- P02790
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途径
- Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
抗原
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