QPCT Protein (AA 33-361) (His tag)
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Quick Overview for QPCT Protein (AA 33-361) (His tag) (ABIN7195939)
抗原
See all QPCT 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 33-361
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标记
- This QPCT protein is labelled with His tag.
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原理
- Recombinant Human Glutaminyl cyclase/QPCT Protein (His Tag)
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序列
- Ala33-Leu361
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产品特性
- A DNA sequence encoding the human QPCT (Q16769-1) (Ala33-Leu361) was fused with a polyhistide tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Glutaminyl-Peptide Cyclotransferase (QPCT) (AA 1-361) protein (GST tag)
QPCT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Glutaminyl-Peptide Cyclotransferase (QPCT) (AA 29-361) protein (His-SUMO Tag)QPCT 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. SDS
Glutaminyl-Peptide Cyclotransferase (QPCT) protein (Myc-DYKDDDDK Tag)QPCT 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- QPCT (Glutaminyl-Peptide Cyclotransferase (QPCT))
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别名
- Glutaminyl cyclase/QPCT
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背景
- GCT,QC,sQC,Glutaminyl cyclase, also known as QPCT, can promote the N-terminal cyclization reaction of N-terminal pyroglutamate(pGlu). The pGlu formation from its glutaminyl precursor is required in the maturation of numerous bioactive peptides, while the aberrant formation of pGlu may be related to several pathological processes, such as osteoporosis and amyloidotic diseases. Glutaminyl cyclase's structure reveals an alpha/beta scaffold akin to that of two-zinc exopeptidases but with several insertions and deletions, particularly in the active-site region. Glutaminyl cyclase's amino acid sequence of this enzyme is 86 % identical to that of bovine glutaminyl cyclase. It is responsible for the presence of pyroglutamyl residues in many neuroendocrine peptides.
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分子量
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Calculated MW: 39.7 kDa
Observed MW: 38 kDa
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基因ID
- 25797
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UniProt
- Q16769
抗原
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