Fibronectin 蛋白
Quick Overview for Fibronectin 蛋白 (ABIN7318495)
抗原
See all Fibronectin 蛋白蛋白类型
生物活性
宿主
资源
纯度
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原理
- Recombinant Human Fibronectin/FN Protein (Active)
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序列
- Pro1270-Ser1546&Ala1721-Thr2016
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产品特性
- Recombinant Human Fibronectin fragment is produced by our E.coli expression system and the target gene encoding Pro1270-Ser1546&Ala1721-Thr2016 is expressed.
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内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
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Biological Activity Comment
- Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is 0.1-0.5 ug/ml.
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Fibronectin (AA 1087-1172) protein (His tag)
宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Fibronectin (AA 1008-1231) protein (His-IF2DI Tag)宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from a 0.2 μm filtered solution of 12.5 mM Sodium Citrate, 1.25 % Sucrose, pH 6.2.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Fibronectin
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别名
- Fibronectin/FN
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背景
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Background: Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains,fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment, is a protein of ~63 kDa containing a central cell-binding domain, a high affinity heparin-binding domain II,and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand, a CS-I site, and a VLA-5 ligand, a cell attachment domain, and virus vectors binds to a heparin binding domain II, which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors, and facilitates the gene transduction in the result.
Synonym: Fibronectin, FN1, CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF
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分子量
- 62.7 kDa
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UniProt
- P02751
抗原
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