Fibronectin Protein (AA 1270-1546, AA 1721-2016)
This Recombinant Fibronectin protein is expressed in 大肠杆菌（E. Coli）.
产品编号 ABIN7318495
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
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Quick Overview for Fibronectin Protein (AA 1270-1546, AA 1721-2016) (ABIN7318495)
抗原
See all Fibronectin 蛋白
Fibronectin
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
大肠杆菌（E. Coli）
应用范围
Cell Culture (CC)
纯度
> 95 % as determined by reducing SDS-PAGE.
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产品特性
- AA 1270-1546, AA 1721-2016
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原理
- Recombinant Human Fibronectin/FN Protein
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序列
- Pro1270-Ser1546&Ala1721-Thr2016
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产品特性
- Recombinant Human Fibronectin fragment is produced by our E.coli expression system and the target gene encoding Pro1270-Ser1546&Ala1721-Thr2016 is expressed.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 0.01 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is 0.1-0.5 μg/ml.
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from a 0.2 μm filtered solution of 12.5 mM Citric acid, 1.25 % Sucrose, 0.1 % Tween 80, pH 5.5 .
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- Fibronectin
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别名
- Fibronectin/FN
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背景
- CIG,ED-B,FINC,FN,FN1,FNZ,Fibronectin,GFND,GFND2,LETS,MSF,Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains,fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment, is a protein of ~63 kDa containing a central cell-binding domain, a high affinity heparin-binding domain II,and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand, a CS-I site, and a VLA-5 ligand, a cell attachment domain, and virus vectors binds to a heparin binding domain II, which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors, and facilitates the gene transduction in the result.
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分子量
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Calculated MW: 62.7 kDa
Observed MW: 60-80 kDa
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基因ID
- 2335
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UniProt
- P02751
抗原
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