Quick Overview for EPOR Protein (His tag) (ABIN7320425)
抗原
蛋白类型
生物活性
宿主
资源
纯度
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标记
- This EPOR protein is labelled with His tag.
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原理
- Recombinant Mouse EPO Receptor/EPOR Protein (His Tag)(Active)
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序列
- Met 1-Pro 249
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产品特性
- A DNA sequence encoding the extracellular domain of mouse EPOR (NP_034279.3) (Met 1-Pro 249) was expressed, with a C-terminal polyhistidine tag.
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- 1. Measured by its ability to inhibit EPO-dependent proliferation of TF-1 human erythroleukemic cells.The ED50 for this effect is typically 0.1-0.5 μg/mL in the presence of 16 ng/mL Recombinant mouse EPO.2. Measured by its binding ability in a functional ELISA.3. Immobilized mouse EPOR-His at 10μg/mL (100μL/well) can bind biotinylated mouse EPO-His.The EC50 of biotinylated mouse EPO-His is 34.5-80.6ng/mL.
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Erythropoietin Receptor (EPOR) (AA 25-138) protein (His-IF2DI Tag)
EPOR 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Erythropoietin Receptor (EPOR) (AA 1-508) protein (GST tag)EPOR 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Erythropoietin Receptor (EPOR) (AA 25-250) protein (His tag)EPOR 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 95% by SDS-PAGE SDS
Erythropoietin Receptor (EPOR) (AA 25-250) (Active) protein (His tag)EPOR 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 95% by SDS-PAGE SDS, AcA Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from sterile PBS, pH 7.4
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- EPOR (Erythropoietin Receptor (EPOR))
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别名
- EPO Receptor/EPOR
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背景
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Background: Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis, and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor (EPOR/EPO Receptor) on bone marrow erythroblasts, which transmits signals important for both proliferation and differentiation along the erythroid lineage. EPOR protein is a type â… single-transmembrane cytokine receptor, and belongs to the homodimerizing subclass which functions as ligand-induced or ligand-stabilized homodimers. EPOR signaling prevents neuronal death and ischemic injury. Recent studies have shown that EPO and EPOR protein may be involved in carcinogenesis, angiogenesis, and invasion.
Synonym: Epor
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分子量
- 26.2 kDa
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NCBI登录号
- NP_034279
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途径
- JAK/STAT Signaling
抗原
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