Endoglin Protein (ENG) (Fc Tag)
Quick Overview for Endoglin Protein (ENG) (Fc Tag) (ABIN7318005)
抗原
See all Endoglin (ENG) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This Endoglin protein is labelled with Fc Tag.
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原理
- Recombinant Human Endoglin/CD105 Protein (Fc Tag)(Active)
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序列
- Met 1-Gly 586
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产品特性
- A DNA sequence encoding the extracellular domain (Met 1-Gly 586) of human CD105 (NP_001108225.1) precursor was expressed with C-terminal fused human IgG1 Fc region.
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内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
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Biological Activity Comment
- 1. Measured by its ability to bind biotinylated Human TGFRB1 in a functional ELISA.2. Measured by its ability to bind biotinylated Human ALK1-Fc in functional Elisa.
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Endoglin (ENG) (AA 26-586) protein (His-Avi Tag)
ENG 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Endoglin (ENG) (AA 1-658) protein (GST tag)ENG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Endoglin (ENG) (AA 394-581) protein (His-IF2DI Tag)ENG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Endoglin (ENG) (AA 27-658) protein (GST tag)ENG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Endoglin (ENG) (AA 26-586) protein (His tag)ENG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85% by SDS-PAGE SDS
Endoglin (ENG) (AA 27-581) (Active) protein (His tag)ENG 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 95% by SDS-PAGE SDS, AcA Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from sterile PBS, pH 7.4
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Endoglin (ENG)
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别名
- Endoglin/CD105
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背景
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Background: Endoglin, also known as CD105, is a type I homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. Endoglin contains an RGD tripeptide which is a key recognition structure in cellular adhesion,,suggesting a critical role for endoglin in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. As an accessory receptor for the TGF-β superfamily ligands, endoglin binds TGF-β1 and TGF-β3 with high affinity not by itself but by associating with TGF-β type I I receptor (TβRII) and activates the downstream signal pathways. In addition, in human umbilical vein endothelial cells, ALK-1 is also a receptor kinase for endoglin threonine phosphorylation, and mutations in either of the two genes result in the autosomal-dominant vascular dysplasia, hereditary hemorrhagic telangiectasia (HHT). Endoglin has been regarded as a powerful biomarker of neovascularization, and is associated with several solid tumor types.
Synonym: Endoglin; END; CD105; ENG;HHT1;ORW1
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分子量
- 87.5 kDa
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NCBI登录号
- NP_001108225
抗原
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