EED Protein (His-GST)
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Quick Overview for EED Protein (His-GST) (ABIN7317586)
抗原
See all EED 蛋白蛋白类型
宿主
资源
纯度
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标记
- This EED protein is labelled with His-GST.
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原理
- Recombinant Human EED/Embryonic Ectoderm Development Protein (His & GST Tag)
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序列
- Met 1-Arg 441
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产品特性
- A DNA sequence encoding the human EED (O75530-1) (Met 1-Arg 441) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Embryonic Ectoderm Development (EED) (AA 1-441) protein (GST tag)
EED 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Embryonic Ectoderm Development (EED) (AA 1-441) protein (His tag)EED 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Embryonic Ectoderm Development (EED) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)EED 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Embryonic Ectoderm Development (EED) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)EED 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 2 mM GSH, 10 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- EED (Embryonic Ectoderm Development (EED))
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别名
- EED/Embryonic Ectoderm Development
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背景
- HEED,WAIT1,Clusterin, also known as complement-associated protein SP-40, Complement cytolysis inhibitor, Apolipoprotein J, Testosterone-repressed prostate message 2, Aging-associated gene 4 protein, CLU and APOJ, is a secreted protein which belongs to the clusterin family. Clusterin/Apolipoprotein J/Apo-J is an enigmatic glycoprotein with a nearly ubiquitous tissue distribution and an apparent involvement in biological processes ranging from mammary gland involution to neurodegeneration in Alzheimer's disease. Its major form, a heterodimer, is secreted and present in physiological fluids, but truncated forms targeted to the nucleus have also been identified. Clusterin/Apolipoprotein J/Apo-J is a widely distributed glycoprotein with a wide range of biologic properties. A prominent and defining feature of clusterin is its marked induction in such disease states as glomerulonephritis, cystic renal disease, renal tubular injury, neurodegenerative conditions, atherosclerosis, and myocardial infarction. Upregulation of clusterin mRNA and protein levels detected in diverse disease states and in in vitro systems have led to suggestions that it functions in membrane lipid recycling, in apoptotic cell death, and as a stress-induced secreted chaperone protein, amongst others.
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分子量
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Calculated MW: 78.0 kDa
Observed MW: 75-85 kDa
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基因ID
- 8726
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UniProt
- O75530
抗原
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