DCLK1 Protein (AA 1-705) (His-GST)
This Recombinant DCLK1 protein is expressed in Baculovirus infected Insect Cells.
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产品编号 ABIN7317068
发货至:
中国
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Quick Overview for DCLK1 Protein (AA 1-705) (His-GST) (ABIN7317068)
抗原
See all DCLK1 蛋白
DCLK1
(Doublecortin-Like Kinase 1 (DCLK1))
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
Baculovirus infected Insect Cells
纯度
> 84 % as determined by reducing SDS-PAGE.
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产品特性
- AA 1-705
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标记
- This DCLK1 protein is labelled with His-GST.
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原理
- Recombinant Human DCAMKL1 Protein (aa 1-705, His & GST Tag)
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序列
- Met 1-Val 705
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产品特性
- A DNA sequence encoding the human DCLK1 (O15075-1) (Met 1-Val 705) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- The specific activity was determined to be 6. 1 nmol/min/mg using synthetic Autocamtide-2 peptide (KKALRRQETVDAL-amide) as substrate.
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Doublecortin-Like Kinase 1 (DCLK1) (AA 640-729) protein (GST tag)
DCLK1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Doublecortin-Like Kinase 1 (DCLK1) (AA 586-740) protein (His-IF2DI Tag)DCLK1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol, 0.5 mM PMSF
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储存液
- PMSF
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储存条件
- -20 °C
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储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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有效期
- 6 months
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- DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))
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别名
- DCAMKL1
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背景
- CL1,CLICK1,DCAMKL1,DCDC3A,DCLK,DCAMKL1, also known as DCLK1, is a member of the protein kinase superfamily and the doublecortin family. It contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. DCAMKL1 is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. Its microtubule-polymerizing activity is independent of its protein kinase activity. DCAMKL1 may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. It may also participate in functions of the mature nervous system.
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分子量
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Calculated MW: 106 kDa
Observed MW: 105 kDa
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基因ID
- 9201
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UniProt
- O15075
抗原
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