GM-CSF 蛋白
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北京 101111
Quick Overview for GM-CSF 蛋白 (ABIN7318085)
抗原
See all GM-CSF (CSF2) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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原理
- Recombinant Human GM-CSF/CSF2 Protein (HEK293 Cells)
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序列
- Met 1-Glu144
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产品特性
- A DNA sequence encoding human GMCSF (NP_000749.2) (Met1-Glu144) was expressed.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.6 ng/ml.
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Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) protein (Fc Tag)
CSF2 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-141) (Active) proteinCSF2 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-141) (Active) protein (His tag)CSF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 98 % as determined by reducing SDS-PAGE. CC Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-141) (Active) protein (His tag)CSF2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE. CC Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-141) (Active) protein (His tag)CSF2 宿主: 小鼠 宿主: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, ELISA, Imm, AcA Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) protein (His tag)CSF2 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 1-144) protein (GST tag)CSF2 宿主: 人 宿主: 小麦胚 Recombinant AP, AA, ELISA, WB
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) (Active) protein (His tag)CSF2 宿主: 人 宿主: HEK-293 Cells > 95 % AcA, CC Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
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别名
- GM-CSF/CSF2
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背景
- CSF,CSF2,Colony-Stimulating Factor,GM-CSF,GMCSF,Granulocyte-Macrophage Colony-Stimulating Factor,Molgramostin,Sargramostim,Granulocyte-macrophage colony-stimulating factor (GM-CSF) is one of an array of cytokines with pivotal roles in embryo implantation and subsequent development. Several cell lineages in the reproductive tract and gestational tissues synthesise GM-CSF under direction by ovarian steroid hormones and signalling agents originating in male seminal fluid and the conceptus. The pre-implantation embryo, invading placental trophoblast cells and the abundant populations of leukocytes controlling maternal immune tolerance are all subject to GM-CSF regulation. GM-CSF stimulates the differentiation of hematopoietic progenitors to monocytes and neutrophils, and reduces the risk for febrile neutropenia in cancer patients. GM-CSF also has been shown to induce the differentiation of myeloid dendritic cells (DCs) that promote the development of T-helper type 1 immune responses in cognate T cells. As a part of the immune/inflammatory cascade, GM-CSF promotes Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity, and thus worthy of consideration for therapeutic target. GM-CSF has been utilized in the clinical management of multiple disease processes. Most recently, GM-CSF has been incorporated into the treatment of malignancies as a sole therapy, as well as a vaccine adjuvant. While the benefits of GM-CSF in this arena have been promising, recent reports have suggested the potential for GM-CSF to induce immune suppression and, thus, negatively impact outcomes in the management of cancer patients.
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分子量
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Calculated MW: 14.5 kDa
Observed MW: 23.8 kDa
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基因ID
- 1437
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NCBI登录号
- NP_000749
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UniProt
- P04141
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途径
- JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin
抗原
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