CD97 Protein (CD97) (Fc Tag)
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北京 101111
Quick Overview for CD97 Protein (CD97) (Fc Tag) (ABIN7317596)
抗原
See all CD97 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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标记
- This CD97 protein is labelled with Fc Tag.
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原理
- Recombinant Human CD97 Protein (Fc Tag)
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序列
- Met 1-Gln 398
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产品特性
- A DNA sequence encoding the first 398 amino acids (Met 1-Gln 398) of human CD97 isoform 2 (NP_001775.2) extracellular domain was fused with the Fc region of human IgG1 at the C-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Immobilized human CD55 at 2 μg/ml (100 μl/well) can bind human CD97 with a linear ranger of 1.28-32 ng/ml.
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CD97 (CD97) (AA 1-742) protein (GST tag)
CD97 宿主: 人 宿主: 小麦胚 Recombinant AP, AA, ELISA, WB
CD97 (CD97) protein (His tag)CD97 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS
CD97 (CD97) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)CD97 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- CD97
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别名
- CD97
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背景
- CD97,TM7LN1,The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. The CD97 is a receptor predominantly expressed in leukocytes and belongs to a new group of seven-span transmembrane molecules, which is also designed EGF-TM7 family. The family members are characterized by an extended extracellular region with several N-terminal epidermal growth factor-like domains two of which contain a calcium binding site. Muture CD 97 has two noncovalently associated subunits and is composed of a large extracellular protein (CD97 alpha) and a seven-membrane spanning protein (CD97 beta). CD97 is considered as a defining feature of G protein-coupled receptors. The effects that lymphocytes and erythrocytes adere to CD97-transfected COS cells suggest that CD97 has the ability to bind cellular ligands. CD97 alpha has three alternatively spliced isforms that are related to the calium binding EGF-like repeats in the microfibril protein fibrillin. Leukocytes strongly positive for CD97 are concentrated at sites of inflammation relative to CD97 expression in normal lymphoid tissues.
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分子量
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Calculated MW: 68.2 kDa
Observed MW: 100-110 kDa
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基因ID
- 976
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NCBI登录号
- NP_001775
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UniProt
- P48960
抗原
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