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Quick Overview for CD6 Protein (CD6) (His tag) (ABIN7194912)
抗原
See all CD6 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This CD6 protein is labelled with His tag.
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原理
- Recombinant Rat CD6/TP120 Protein (His Tag)(Active)
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序列
- Met1-Asn396
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产品特性
- A DNA sequence encoding the rat CD6 (Q812A4) (Met1-Asn396) was expressed with a polyhistidine tag at the C-terminus.
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method
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Biological Activity Comment
- 1. Immobilized rat CD6-His at 10 μg/mL (100 μL/well) can bind rat ALCAM-Fc3 , The EC50 of rat ALCAM-Fc3 is 19.5-45.5 ng/ml. 2. Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.When 8 x 104 cells/well are added to ratCD6-His coated plates (10μg/mL, 100 μL/well), approximately 39.2% will adhere after 60 minutes at 37°C.
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CD6 (CD6) (AA 18-402) protein (His tag)
CD6 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD6 (CD6) protein (His tag)CD6 宿主: 小鼠 宿主: 人细胞 Recombinant > 90 % as determined by reducing SDS-PAGE.
CD6 (CD6) (Active) protein (His tag)CD6 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
CD6 (CD6) (Active) protein (Fc Tag)CD6 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE Active
CD6 (CD6) (Active) protein (Fc Tag)CD6 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE. Active
CD6 (CD6) (AA 308-400) protein (GST tag)CD6 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
CD6 (CD6) (AA 18-398) protein (His tag)CD6 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from sterile PBS, pH 7.4
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- CD6
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别名
- CD6/TP120
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背景
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Background: T-cell differentiation antigen CD6, also known as TP120 and CD6, is a single-pass type I membrane protein which contains three SRCR domains. CD6 / TP120 is a cell surface glycoprotein expressed primarily on T cells, it may function as a costimulatory molecule and may play a role in autoreactive immune responses. CD6 / TP120 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. CD6 ligand termed CD166 (previously known as activated leukocyte cell adhesion molecule, ALCAM ) has been identified and shown to be expressed on activated T cells, B cells, thymic epithelium, keratinocytes, and in rheumatoid arthritis synovial tissue. CD6 / TP120 binds to activated leukocyte cell adhesion molecule ( CD166 ), and is considered as a costimulatory molecule involved in lymphocyte activation and thymocyte development. CD6 / TP120 partially associates with the TCR / CD3 complex and colocalizes with it at the center of the mature immunological synapse (IS) on T lymphocytes. During thymic development CD6-dependent signals may contribute both to thymocyte survival, and to the overall functional avidity of selection in both man and mouse.
Synonym: CD6
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分子量
- 42.5 kDa
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UniProt
- Q812A4
抗原
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