CD47 Protein (CD47) (Fc Tag)
Quick Overview for CD47 Protein (CD47) (Fc Tag) (ABIN7194872)
抗原
See all CD47 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This CD47 protein is labelled with Fc Tag.
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原理
- Recombinant Human CD47 Protein (Fc Tag)(Active)
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序列
- Met 1-Pro139
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产品特性
- A DNA sequence encoding the human CD47 (NP_942088.1) (Met1-Pro139) was expressed with the Fc region of human IgG1 at the C-terminus.
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内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
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Biological Activity Comment
- 1. Measured by its binding ability in a functional ELISA. Immobilized human SIRPA-His at 10 μg/ml (100 μl/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 10.1-23.5 ng/ml.2. Measured by its binding ability in a functional ELISA. Immobilized mouse SIRPA-His at 10 μg/ml (100 μl/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 0.05-0.13 μg/ml.3. Measured by its binding ability in a functional ELISA. Immobilized human SIRPG-His at 10 μg/ml (100 μl/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 0.58-1.34 μg/ml.
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CD47 (CD47) (AA 19-139) protein (Fc Tag)
CD47 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD47 (CD47) (AA 19-139) protein (His tag)CD47 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD47 (CD47) (AA 19-141) protein (His tag)CD47 宿主: 猕猴, Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD47 (CD47) (AA 19-139) protein (His-Avi Tag,Biotin)CD47 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD47 (CD47) (AA 19-141) protein (mFc-His Tag)CD47 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
CD47 (CD47) (AA 19-140) protein (His tag)CD47 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD47 (CD47) (AA 19-140) protein (Fc Tag)CD47 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD47 (CD47) (AA 19-139) (Active) protein (Fc Tag)CD47 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD47 (CD47) (AA 19-139) (Active) protein (His tag)CD47 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD47 (CD47) (AA 19-139) (Active) protein (Fc Tag,AVI tag,Biotin)CD47 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from sterile PBS, pH 7.4
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- CD47
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别名
- CD47
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背景
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Background: CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain and is a receptor for the C-terminal cell binding domain of thrombospondin. It may play a role in membrane transport and signal transduction. CD47 is also a membrane protein; which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. It is very broadly distributed on normal adult tissues; as well as ovarian tumors; being especially abundant in some epithelia and the brain. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. It may prevent premature elimination of red blood cells. It also may be involved in membrane permeability changes induced following virus infection. By acting as an adhesion receptor for THBS1 on platelets; CD47 plays a role in both cell adhesion and in the modulation of integrins. It also plays an important role in memory formation and synaptic plasticity in the hippocampus.Immune CheckpointImmune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: WB AntibodiesImmune Checkpoint Targets Co-inhibitory Immune Checkpoint Targets Immunotherapy Cancer Immunotherapy Targeted Therapy
Synonym: IAP;MER6;OA3
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分子量
- 40.7 kDa
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NCBI登录号
- NP_942088
抗原
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