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Quick Overview for CD4 Protein (CD4) (His tag) (ABIN7320016)
抗原
See all CD4 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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标记
- This CD4 protein is labelled with His tag.
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原理
- Recombinant Mouse CD4/LEU3 Protein (His Tag)
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序列
- Met 1-Thr 394
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产品特性
- A DNA sequence encoding the extracellular domain of mouse CD4 (NP_038516.1) (Met 1-Thr 394) was expressed, fused with a polyhistidine tag at the C-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When 5 x 104 cells/well are added to mCD4-His coated plates (0.8μg/mL and 100μL/well), approximately > 50% will adhere specifically after 30 minutes at 37°C.
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CD4 (CD4) (AA 26-390) (Active) protein (His-Avi Tag)
CD4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD4 (CD4) (AA 26-390) (Active) protein (His tag)CD4 宿主: 猕猴 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD4 (CD4) (AA 26-390) (Active) protein (hFc Tag)CD4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD4 (CD4) (AA 26-390) (Active) protein (Biotin,His-Avi Tag)CD4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD4 (CD4) (AA 27-394) (Active) protein (His tag)CD4 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD4 (CD4) (AA 26-390) (Active) protein (Fc Tag)CD4 宿主: 猕猴 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD4 (CD4) (AA 26-396) (Active) protein (Fc Tag,AVI tag,Biotin)CD4 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD4 (CD4) (AA 26-396) (Active) protein (His tag,AVI tag,Biotin)CD4 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD4 (CD4) (AA 26-390) (Active) protein (His tag)CD4 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD4 (CD4) (AA 26-396) (Active) protein (Fc Tag)CD4 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- CD4
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别名
- CD4
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背景
- L3T4,Ly-4,T-cell surface glycoprotein CD4, is a single-pass type I membrane protein. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. The CD4 surface determinant, previously associated as a phenotypic marker for helper/inducer subsets of T lymphocytes, has now been critically identified as the binding/entry protein for human immunodeficiency viruses (HIV). The human CD4 Molecule is readily detectable on monocytes, T lymphocytes, and brain tissues. CD4 is a co-receptor that assists the T cell receptor (TCR) to activate its T cell following an interaction with an antigen presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR. CD4 interacts directly with MHC class II molecules on the surface of the antigen presenting cell via its extracellular domain. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Viral protein U (VpU) of HIV-1 plays an important role in downregulation of the main HIV-1 receptor CD4 from the surface of infected cells. Physical binding of VpU to newly synthesized CD4 in the endoplasmic reticulum is an early step in a pathway leading to proteasomal degradation of CD4.
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分子量
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Calculated MW: 42.8 kDa
Observed MW: 50-55 kDa
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基因ID
- 12504
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NCBI登录号
- NP_038516
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UniProt
- P06332
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途径
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
抗原
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