CD33 Protein (CD33) (His tag)
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Quick Overview for CD33 Protein (CD33) (His tag) (ABIN7194814)
抗原
See all CD33 蛋白蛋白类型
宿主
资源
纯度
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标记
- This CD33 protein is labelled with His tag.
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原理
- Recombinant Human SIGLEC3/CD33 Protein (His Tag)
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序列
- Met 1-His259
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产品特性
- A DNA sequence encoding the human CD33 (AAH28152.1) (Met1-His259) was expressed with a C-terminal polyhistidine tag.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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CD33 (CD33) (AA 18-259) (Active) protein (His-Avi Tag)
CD33 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD33 (CD33) (AA 18-259) (Active) protein (hFc Tag)CD33 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD33 (CD33) (AA 18-259) (Active) protein (Biotin,His-Avi Tag)CD33 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD33 (CD33) (AA 18-259) protein (His tag,Fc Tag)CD33 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, AM, ADA, IA
CD33 (CD33) (AA 17-248) (Active) protein (His tag)CD33 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
CD33 (CD33) (AA 18-259) (Active) protein (His-Fc tag)CD33 宿主: 人 宿主: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. ELISA, WB, SDS, Imm, AcA Active
CD33 (CD33) (AA 18-240) protein (Fc Tag)CD33 宿主: 小鼠 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, AM, ADA, IA
CD33 (CD33) (AA 140-259) protein (Fc Tag)CD33 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, AM, ADA, IA
CD33 (CD33) (AA 18-259) (Active) protein (His-Avi Tag,FITC)CD33 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
CD33 (CD33) (AA 18-259) protein (His tag)CD33 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile PBS, pH 7.4, 10 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- CD33
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别名
- SIGLEC3/CD33
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背景
- CD33,Myeloid Cell Surface Antigen CD33,SIGLEC3,Sialic Acid-Binding Ig-Like Lectin 3,Siglec-3,gp67,Myeloid cell surface antigen CD33 also known as Sialic acid binding Ig-like lectin 3, CD33 antigen or Siglec-3, is a member of the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. This Single-pass type I membrane protein contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain. CD33 /Siglec-3 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. CD33 /Siglec-3 preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33/Siglec-3 induces apoptosis in acute myeloid leukemia (in vitro). CD33/Siglec-3 can function as a sialic acid-dependent cell adhesion molecule and that binding can be modulated by endogenous sialoglycoconjugates when CD33 is expressed in a plasma membrane.
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分子量
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Calculated MW: 28.2 kDa
Observed MW: 40-46 kDa
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基因ID
- 945
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UniProt
- P20138
抗原
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