CD16 Protein (CD16) (His tag)
This Recombinant CD16 protein is expressed in HEK-293 Cells.
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产品编号 ABIN7194703
发货至:
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Quick Overview for CD16 Protein (CD16) (His tag) (ABIN7194703)
抗原
See all CD16 蛋白
CD16
蛋白类型
Recombinant
生物活性
Active
宿主
小鼠
资源
HEK-293 Cells
应用范围
ELISA
纯度
> 95 % as determined by reducing SDS-PAGE.
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标记
- This CD16 protein is labelled with His tag.
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原理
- Recombinant Mouse CD16 Protein (His Tag)
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序列
- Met 1-Thr 215
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产品特性
- A DNA sequence encoding the mouse FCGR3 (P08508-1) extracellular domain (Met 1-Thr 215) was fused with a polyhistidine tag at the C-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Immobilized mouse FCGR3-His at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1The EC50 of biotinylated human IgG1 is 0.12-0.28 μg/ml.
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CD16 (CD16) (AA 32-215) protein (His tag)
CD16 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
CD16 (CD16) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)CD16 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- CD16
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别名
- CD16
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背景
- CD16 Protein;Mouse,Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.
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分子量
- 22.6 kDa
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UniProt
- P08508
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
抗原
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