CD16 Protein (CD16) (His tag)
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Quick Overview for CD16 Protein (CD16) (His tag) (ABIN7194703)
抗原
See all CD16 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This CD16 protein is labelled with His tag.
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原理
- Recombinant Mouse CD16 Protein (His Tag)(Active)
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序列
- Met 1-Thr 215
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产品特性
- A DNA sequence encoding the mouse FCGR3 (P08508-1) extracellular domain (Met 1-Thr 215) was fused with a polyhistidine tag at the C-terminus.
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Measured by its binding ability in a functional ELISA.Immobilized mouse FCGR3-His at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1The EC50 of biotinylated human IgG1 is 0.12-0.28 μg/ml.
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CD16 (CD16) protein (His tag)
CD16 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
CD16 (CD16) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)CD16 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from sterile PBS, pH 7.4
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- CD16
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别名
- CD16
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背景
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Background: Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.
Synonym: CD16 Protein, Mouse
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分子量
- 22.6 kDa
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
抗原
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