CMBL Protein (His tag)
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- 抗原 See all CMBL 蛋白
- CMBL (Carboxymethylenebutenolidase Homolog (CMBL))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This CMBL protein is labelled with His tag.
- 原理
- Recombinant Human CMBL Protein (His Tag)
- 序列
- Met 1-Met 245
- 产品特性
- A DNA sequence encoding the human CMBL (Q96DG6) (Met 1-Met 245) was expressed, with a N-terminal polyhistidine tag.
- 纯度
- > 97 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product CMBL 蛋白
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Carboxymethylenebutenolidase Homolog (CMBL) (AA 1-245) protein (His tag)
CMBL 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Carboxymethylenebutenolidase Homolog (CMBL) (AA 1-245) protein (GST tag)CMBL 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Carboxymethylenebutenolidase Homolog (CMBL) (AA 2-245) protein (His tag)Crystallography grade CMBL 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Carboxymethylenebutenolidase Homolog (CMBL) (AA 2-245) protein (His tag)Crystallography grade CMBL 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Carboxymethylenebutenolidase Homolog (CMBL) protein (Myc-DYKDDDDK Tag)CMBL 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 20 mM Tris, 0.1 % Brij35, pH 8.0
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- CMBL (Carboxymethylenebutenolidase Homolog (CMBL))
- 别名
- CMBL (CMBL 产品)
- 别名
- JS-1 Protein, RGD1306952 Protein, 2310016A09Rik Protein, carboxymethylenebutenolidase homolog Protein, carboxymethylenebutenolidase-like (Pseudomonas) Protein, CMBL Protein, Cmbl Protein
- 背景
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Background: Carboxymethylenebutenolidase (CMBL), also known as 4-carboxymethylenebut-2-en-4-olide lactonohydrolase, maleylacetate enol- lactonase, dienelactone hydrolase, and carboxymethylene butenolide hydrolase, is a hydrolase specially belonging to the family of hydrolases. It maily acts on carboxylic ester bonds. CMBL is a human homolog of Pseudomonas dienelactone hydrolase involved in the bacterial halocatechol degradation pathway. The ubiquitous expression of human CMBL gene transcript in various tissues was observed. CMBL was demonstrated to be the primary olmesartan medoxomil (OM) bioactivating enzyme in the liver and intestine. The recombinant human CMBL expressed in mammalian cells was clearly shown to activate OM. The recombinant CMBL also converted other prodrugs having the same ester structure as OM, faropenem medoxomil and lenampicillin, to their active metabolites. CMBL exhibited a unique sensitivity to chemical inhibitors, thus, being distinguishable from other known esterases.
Synonym: JS-1
- 分子量
- 30 kDa
- UniProt
- Q96DG6
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