CMBL Protein (His tag)
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北京 101111
Quick Overview for CMBL Protein (His tag) (ABIN7317566)
抗原
See all CMBL 蛋白蛋白类型
宿主
资源
纯度
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标记
- This CMBL protein is labelled with His tag.
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原理
- Recombinant Human CMBL Protein (His Tag)
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序列
- Met 1-Met 245
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产品特性
- A DNA sequence encoding the human CMBL (Q96DG6) (Met 1-Met 245) was expressed, with a N-terminal polyhistidine tag.
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过滤
- 0.2 μm filtered
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Biological Activity Comment
- Not validated for activity
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Carboxymethylenebutenolidase Homolog (CMBL) (AA 1-245) protein (His-IF2DI Tag)
CMBL 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Carboxymethylenebutenolidase Homolog (CMBL) (AA 1-245) protein (GST tag)CMBL 宿主: 人 宿主: 小麦胚 Recombinant AP, AA, ELISA, WB
Carboxymethylenebutenolidase Homolog (CMBL) protein (Myc-DYKDDDDK Tag)CMBL 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile 20 mM Tris, 0.1 % Brij35, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- CMBL (Carboxymethylenebutenolidase Homolog (CMBL))
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别名
- CMBL
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背景
- JS-1,Carboxymethylenebutenolidase (CMBL), also known as 4-carboxymethylenebut-2-en-4-olide lactonohydrolase, maleylacetate enol- lactonase, dienelactone hydrolase, and carboxymethylene butenolide hydrolase, is a hydrolase specially belonging to the family of hydrolases. It maily acts on carboxylic ester bonds. CMBL is a human homolog of Pseudomonas dienelactone hydrolase involved in the bacterial halocatechol degradation pathway. The ubiquitous expression of human CMBL gene transcript in various tissues was observed. CMBL was demonstrated to be the primary olmesartan medoxomil (OM) bioactivating enzyme in the liver and intestine. The recombinant human CMBL expressed in mammalian cells was clearly shown to activate OM. The recombinant CMBL also converted other prodrugs having the same ester structure as OM, faropenem medoxomil and lenampicillin, to their active metabolites. CMBL exhibited a unique sensitivity to chemical inhibitors, thus, being distinguishable from other known esterases.
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分子量
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Calculated MW: 30.0 kDa
Observed MW: 28 kDa
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基因ID
- 134147
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UniProt
- Q96DG6
抗原
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