Calsequestrin Protein (AA 35-396)
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Quick Overview for Calsequestrin Protein (AA 35-396) (ABIN7317197)
抗原
See all Calsequestrin (CASQ1) 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 35-396
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原理
- Recombinant Human Calsequestrin-1/CASQ1 Protein
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序列
- Gln35-Asp396
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产品特性
- A DNA sequence encoding the mature form of human CASQ1 (P31415) (Gln35-Asp396) was expressed with a N-terminal Met.
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过滤
- 0.2 μm filtered
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Biological Activity Comment
- Not validated for activity
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Calsequestrin 1 (CASQ1) (AA 1-390) protein (GST tag)
CASQ1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Calsequestrin 1 (CASQ1) protein (Myc-DYKDDDDK Tag)CASQ1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Calsequestrin 1 (CASQ1) proteinCASQ1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant WB, SDS, ELISA, IP
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile 50 mM Tris, 10 % glycerol, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- Calsequestrin (CASQ1) (Calsequestrin 1 (CASQ1))
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别名
- Calsequestrin-1/CASQ1
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背景
- CASQ,PDIB1,VMCQA,Calsequestrin-1 is an isoform of calsequestrin. Calsequestrin is a calcium-binding protein of the sarcoplasmic reticulum. It helps hold calcium in the cisterna of the sarcoplasmic reticulum after a muscle contraction, even though the concentration of calcium in the sarcoplasmic reticulum is much higher than in the cytosol. Two forms of calsequestrin have been identified: Calsequestrin-2 and Calsequestrin-1. Calsequestrin-1 is found in fast skeletal muscle. The release of calsequestrin-bound calcium (through a calcium release channel) triggers muscle contraction. The active protein is not highly structured, more than 50 % of it adopting a random coil conformation. When calcium binds there is a structural change whereby the alpha-helical content of the protein increases from 3 to 11 %. Both forms of calsequestrin are phosphorylated by casein kinase 2, but the cardiac form is phosphorylated more rapidly and to a higher degree. Calsequestrin-1 is also secreted in the gut where it deprives bacteria of calcium ions.
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分子量
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Calculated MW: 41.8 kDa
Observed MW: 52 kDa
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基因ID
- 844
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UniProt
- P31415
抗原
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