CAMK4 蛋白
Quick Overview for CAMK4 蛋白 (ABIN7320128)
抗原
See all CAMK4 蛋白蛋白类型
宿主
资源
纯度
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原理
- Recombinant Mouse CAMK4/CaMKIV Protein (Active)
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序列
- Met1-Tyr469
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产品特性
- A DNA sequence encoding the mouse CAMK4(P08414) (Met1-Tyr469) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Kinase activity untested
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Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) (AA 1-469) protein (His tag)
custom-made CAMK4 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) (AA 1-473) protein (His tag)custom-made CAMK4 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) (AA 1-473) protein (GST tag)CAMK4 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP, KAA
Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) protein (GST tag,His tag)CAMK4 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 94 % as determined by SDS-PAGE
Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) (AA 1-473) protein (His tag)CAMK4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) protein (Myc-DYKDDDDK Tag)CAMK4 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- CAMK4 (Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4))
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别名
- CAMK4/CaMKIV
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背景
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Background: Ca2+/ calmodulin-dependent protein kinase 4 (CAMKIV) belongs to the serine/threonine protein kinase family, and to the Ca2+/calmodulin-dependent protein kinase subfamily which is widely recognized as an essential enzyme implicated in the phophoinositide amplification cascade. Ca2+/calmodulin dependent protein kinase (CAMK) can be activated by the introcellular increased Ca2+ and then apt to combine with the target protein. Ca2+/ calmodulin-dependent protein kinase 4 (CAMKIV) is a multifunctional CaM-dependent kinase protein with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. All of the isforms of this family, including myosin light chain kinase, phosphorylase kinase, CaMK1, CaMKIII and CaMKIV have EF-hand structure.
Synonym: A430110E23Rik,AI666733,CaMKIV,CaMKIV/Gr,D18Bwg0362e
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分子量
- 52.7 kDa
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UniProt
- P08414
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途径
- EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Production of Molecular Mediator of Immune Response, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma
抗原
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