CAMK4 Protein (AA 1-469) (His-GST)
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Quick Overview for CAMK4 Protein (AA 1-469) (His-GST) (ABIN7320129)
抗原
See all CAMK4 蛋白蛋白类型
宿主
资源
纯度
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产品特性
- AA 1-469
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标记
- This CAMK4 protein is labelled with His-GST.
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原理
- Recombinant Mouse CAMK4/CaMKIV Protein (His & GST Tag)
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序列
- Met1-Tyr469
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产品特性
- A DNA sequence encoding the mouse CAMK4 (P08414) (Met1-Tyr469) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) (AA 1-473) protein (GST tag)
CAMK4 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP, KAA
Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) (AA 1-473) protein (His-IF2DI Tag)CAMK4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) (AA 1-473) protein (His tag)CAMK4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % SDS
Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) (AA 1-469) (Active) proteinCAMK4 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 95 % as determined by reducing SDS-PAGE. Active
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- CAMK4 (Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4))
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别名
- CAMK4/CaMKIV
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背景
- A430110E23Rik,AI666733,CaMKIV,CaMKIV/Gr,D18Bwg0362e,Ca2+/ calmodulin-dependent protein kinase 4 (CAMKIV) belongs to the serine/threonine protein kinase family, and to the Ca2+/calmodulin-dependent protein kinase subfamily which is widely recognized as an essential enzyme implicated in the phophoinositide amplification cascade. Ca2+/calmodulin dependent protein kinase (CAMK) can be activated by the introcellular increased Ca2+ and then apt to combine with the target protein. Ca2+/ calmodulin-dependent protein kinase 4 (CAMKIV) is a multifunctional CaM-dependent kinase protein with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. All of the isforms of this family, including myosin light chain kinase, phosphorylase kinase, CaMK1, CaMKIII and CaMKIV have EF-hand structure.
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分子量
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Calculated MW: 80.4 kDa
Observed MW: 85 kDa
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UniProt
- P08414
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途径
- EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Production of Molecular Mediator of Immune Response, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma
抗原
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