Butyrylcholinesterase Protein (BCHE) (His tag)
-
- 抗原 See all Butyrylcholinesterase (BCHE) 蛋白
- Butyrylcholinesterase (BCHE)
- 蛋白类型
- Recombinant
- 生物活性
- Active
-
宿主
- 人
-
资源
- HEK-293 Cells
- 标记
- This Butyrylcholinesterase protein is labelled with His tag.
- 原理
- Recombinant Human Butyrylcholinesterase/BCHE Protein (His Tag)(Active)
- 序列
- Met 1-Leu 602
- 产品特性
- A DNA sequence encoding the human BCHE (NP_000046.1) (Met 1-Leu 602) was expressed, fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 97 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to cleave Butyrylthiocholine. The specific activity is >50,000 pmoles/min/μg.
- Top Product
- Discover our top product BCHE 蛋白
-
Butyrylcholinesterase (BCHE) (AA 493-602) protein (GST tag)
BCHE 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Butyrylcholinesterase (BCHE) (Active) protein (His tag)BCHE 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE Active
Butyrylcholinesterase (BCHE) (AA 29-602) protein (His tag)Crystallography grade BCHE 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Butyrylcholinesterase (BCHE) (AA 30-603) protein (His tag)Crystallography grade BCHE 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Butyrylcholinesterase (BCHE) (AA 1-141), (full length) protein (His tag)BCHE 宿主: 犬 宿主: 酵母菌 Recombinant > 90 % ELISA
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- 抗原
- Butyrylcholinesterase (BCHE)
- 别名
- Butyrylcholinesterase/BCHE (BCHE 产品)
- 别名
- che Protein, che1 Protein, bche Protein, cholinesterase Protein, CHE1 Protein, CHE2 Protein, E1 Protein, C730038G20Rik Protein, butyrylcholinesterase L homeolog Protein, butyrylcholinesterase Protein, bche.L Protein, bche Protein, BCHE Protein, Bche Protein
- 背景
-
Background: Butyrylcholinesterase (BCHE), also known as cholinesterase or BuChE, is an enzyme defined as "pseudo" or "non-neuronal" cholinesterase. Butyrylcholinesterase (BCHE) is widely distributed in the nervous system as well as blood plasma. It is constitutively similar to the neuronal acetylcholinesterase, and is a non-specific cholinesterase which hydrolyses many different choline esters. Butyrylcholinesterase (BCHE) is a glycoprotein of 4 identical subunits, that were arranged as a dimer of dimers with each dimer composed of two identical subunits joined by interchain disulfide bonds. Butyrylcholinesterase (BCHE) behaves principally similar to the true enzyme and thus can play a similar role in nerve conduction, although it participates probably only in relatively slow conductive processes and could be involved in other nervous system functions and in neurodegenerative diseases. It can hydrolyze toxic esters such as cocaine or scavenge organophosphorus pesticides and nerve agents. Purified human serum cholinesterase combines in its active surface an anionic and an esteratic site, similar to true cholinesterase. It has been demonstrated that butyrylcholinesterase (BCHE) may have a greater role in cholinergic transmission than previously surmised, making BChE inhibition an important therapeutic goal in Alzheimer's disease.
Synonym: Cholinesterase; Acylcholine Acylhydrolase; Butyrylcholine Esterase; Choline Esterase II; Pseudocholinesterase; BCHE; CHE1
- 分子量
- 66.5 kDa
- NCBI登录号
- NP_000046
- 途径
- Peptide Hormone Metabolism
-
