产品编号 ABIN7321012
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for BFAR Protein (AA 1-183) (ABIN7321012)
抗原
See all BFAR 蛋白
BFAR
(Bifunctional Apoptosis Regulator (BFAR))
蛋白类型
Recombinant
宿主
Streptomyces hygroscopicus
资源
大肠杆菌（E. Coli）
纯度
> 95 % as determined by reducing SDS-PAGE.
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产品特性
- AA 1-183
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原理
- Recombinant Streptomyces hygroscopicus Bar Protein
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序列
- Met1-Ile183
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产品特性
- Recombinant Streptomyces hygroscopicus Bar is produced by our E.coli expression system and the target gene encoding Met1-Ile183 is expressed.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Bifunctional Apoptosis Regulator (BFAR) (AA 101-200) protein (GST tag)
BFAR 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Bifunctional Apoptosis Regulator (BFAR) (AA 1-450) protein (GST tag)BFAR 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from a 0.2 μm filtered solution of 12.5 mM Tris-HCl, 50 mM NaCl, 5 % Trehalose, 5 % Mannitol, 0.01 % Tween 80, 2 mM DTT, 1 mM EDTA, pH 8.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存液
- Dithiothreitol (DTT)
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注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- BFAR (Bifunctional Apoptosis Regulator (BFAR))
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别名
- Bar
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背景
- PPT N-acetyltransferase,Phosphinothricin N-acetyltransferase,Phosphinothricin-resistance protein,bar,Phosphinothricin N-acetyltransferase (PAT) is an enzyme that acetylates the free NH2 group of L-phosphinothricin (L-PPT) in the presence of acetyl-CoA as a co-substrate. It is highly specific for L-PPT and does not acetylate other L-amino acids or structurally similar molecules. L-PPT is a glutamate analog that can inhibit glutamine synthetase activity in plants, resulting in the accumulation of ammonia to toxic levels and impairment of photosynthesis. The introduction of a PAT gene into a plant genome can confer resistance to glufosinate herbicide during post-emergent applications.
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分子量
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Calculated MW: 20.6 kDa
Observed MW: 18-20 kDa
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UniProt
- P16426
抗原
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