BACE1 蛋白
Quick Overview for BACE1 蛋白 (ABIN7318053)
抗原
See all BACE1 蛋白蛋白类型
生物活性
宿主
资源
纯度
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原理
- Recombinant Human BACE1/ASP2 Protein (Active)
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序列
- Met 1-Thr 457
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产品特性
- The mature form of human BACE1 (NP_036236.1) extracellular domain (Met1-Thr 457) with a quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified.
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内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
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Biological Activity Comment
- Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dpn)-Arg-Arg-NH2 (Catalog# ES004, R&D Systems). Cleavage of ES004 can be measured using excitation and emission wavelength of 320 and 405 nm, respectively. The specific activity is >3.5 pmoles/min/μg.
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Beta-secretase 1 (BACE1) (AA 22-457) protein (Fc Tag)
BACE1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Beta-secretase 1 (BACE1) (AA 22-457) protein (His tag)BACE1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Beta-secretase 1 (BACE1) (AA 22-457) protein (His tag)BACE1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Beta-secretase 1 (BACE1) (AA 46-152) protein (His-IF2DI Tag)BACE1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Beta-secretase 1 (BACE1) (AA 22-501) protein (GST tag)BACE1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Beta-secretase 1 (BACE1) (Active) protein (His tag)BACE1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
Beta-secretase 1 (BACE1) (AA 22-457) proteinBACE1 宿主: 人 宿主: HEK-293 Cells Recombinant >98 % as determined by SDS-PAGE.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- BACE1 (Beta-secretase 1 (BACE1))
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别名
- BACE1
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背景
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Background: Beta-site APP-cleaving enzyme 1 (BACE1) is an aspartic-acid protease important in the formation of myelin sheaths in peripheral nerve cells. In the brain, This protein is expressed highly in the substantia nigra, locus coruleus and medulla oblongata. Strong BACE1 expression has also been described in pancreatic tissue. BACE1 has a pivotal role in the pathogenesis of Alzheimer's disease. In Alzheimer's disease patients, BACE1 levels were elevated although mRNA levels were not changed. It has been found that BACE1 gene expression is controlled by a TATA-less promoter. The translational repression as a new mechanism controlling its expression. And the low concentrations of Ca(2+) (microM range) significantly increased the proteolytic activity of BACE1. Furthermore, BACE1 protein is ubiquitinated, and the degradation of BACE1 proteins and amyloid precursor protein processing are regulated by the ubiquitin-proteasome pathway. It has also been identified as the rate limiting enzyme for amyloid-beta-peptide (Abeta) production.
Synonym: ASP2,BACE,HSPC104
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分子量
- 49 kDa
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NCBI登录号
- NP_036236
抗原
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