alpha 2 Macroglobulin Protein (His tag)
Quick Overview for alpha 2 Macroglobulin Protein (His tag) (ABIN7317712)
抗原
See all alpha 2 Macroglobulin (A2M) 蛋白蛋白类型
生物活性
宿主
资源
纯度
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标记
- This alpha 2 Macroglobulin protein is labelled with His tag.
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原理
- Recombinant Human A2M/CPAMD5/Alpha-2-macroglobulin Protein (His Tag)(Active)
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序列
- Met 1-Ala 1474
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产品特性
- A DNA sequence encoding the human A2M (NP_000005.2) (Met 1-Ala 1474) was expressed, fused with a polyhistidine tag at the C-terminus.
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内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
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Biological Activity Comment
- Measured by its ability to trap trypsin. The trapped trypsin is no longer able to interact with protein substrates or inhibitors, but still able to cleave small peptide substrates or inhibitors.The IC50 value is <5 nM.
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alpha-2-Macroglobulin (A2M) (AA 641-730) protein (GST tag)
A2M 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please refer to the printed manual for detailed information.
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缓冲液
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))
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别名
- A2M/CPAMD5/Alpha-2-macroglobulin
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背景
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Background: alpha-2-macroglobulin, also known as α2-macroglobulin (α2M and A2M), is an abundant protein of the plasma of vertebrates and members of several invertebrate phyla and functions as a broad-spectrum protease-binding protein. alpha-2-macroglobulin is produced by the liver, and is a major component of the alpha-2 band in protein electrophoresis. alpha-2-macroglobulin is a large plasma glycoprotein that has long been known as an irreversible inhibitor of a variety of proteinases. More recently, it has been reported that numerous growth factors, cytokines and hormones bind to alpha 2M through diverse mechanisms. A2M is also produced in the brain where it binds multiple extracellular ligands and is internalized by neurons and astrocytes. In the brain of Alzheimer's disease (AD) patients, A2M has been localized to diffuse amyloid plaques. A2M also binds soluble beta-amyloid, of which it mediates degradation. Protease-conjugated alpha2-macroglobulin is selectively bound by cells contacting the body fluids and alpha2-macroglobulin and its protease cargo are then internalized and degraded in secondary lysosomes of those cells. In addition to this function as an agent for protease clearance, alpha2-macroglobulin binds a variety of other ligands, including several peptide growth factors and modulates the activity of a lectin-dependent cytolytic pathway in arthropods.
Synonym: A2MD;CPAMD5;FWP007;S863-7
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分子量
- 164 kDa
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NCBI登录号
- NP_000005
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途径
- Lipid Metabolism
抗原
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