Fetuin A Protein (His tag)
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Quick Overview for Fetuin A Protein (His tag) (ABIN7317918)
抗原
See all Fetuin A (AHSG) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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标记
- This Fetuin A protein is labelled with His tag.
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原理
- Recombinant Human Fetuin-A/AHSG Protein (His Tag)
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序列
- Met 1-Val 367
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产品特性
- A DNA sequence encoding the human Fetuin A (NP_001613.2) (Met 1-Val 367) precursor was expressed with a C-terminal polyhistidine tag.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- 1. Immobilized human FCN1 at 10 μg/ml can bind biotinylated recombinant human Fetuin-A with a linear range of 16-2000 ng/ml. 2. Measured by its ability to inhibit active Cathepsin V cleavage of a fluorogenic peptide substrate Z-LR-AMC, R&D Systems, Catalog # ES008.The IC50 value is < 100 nM.
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alpha-2-HS-Glycoprotein (AHSG) (AA 19-367) protein (GST tag)
AHSG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
alpha-2-HS-Glycoprotein (AHSG) (AA 19-367) protein (His tag)AHSG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
alpha-2-HS-Glycoprotein (AHSG) (AA 173-367) protein (His-IF2DI Tag)AHSG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
alpha-2-HS-Glycoprotein (AHSG) (AA 18-367) protein (His-IF2DI Tag)AHSG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
alpha-2-HS-Glycoprotein (AHSG) (AA 19-345) protein (His tag)AHSG 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
alpha-2-HS-Glycoprotein (AHSG) (AA 19-367) protein (His tag)AHSG 宿主: 猕猴 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
alpha-2-HS-Glycoprotein (AHSG) (Active) protein (His tag)AHSG 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE. ELISA Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile PBS, pH 7.2
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- Fetuin A (AHSG) (alpha-2-HS-Glycoprotein (AHSG))
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别名
- Fetuin-A/AHSG
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背景
- AHSG,Alpha-2-HS-Glycoprotein,Alpha-2-Z-Globulin,Ba-Alpha-2-Glycoprotein,FETUA,Fetuin-A,Fetuin-A, also known as Alpha-2-HS-Glycoprotein (AHSG), belongs to the Fetuin family, is a plasma binding protein, and is more abundant in fetal than adult blood. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. Fetuins are carrier proteins like albumin. Fetuin-A forms soluble complexes with calcium and phosphate and thus is a carrier of otherwise insoluble calcium phosphate. Thus Fetuin-A is a potent inhibitor of pathological calcification. The circulating levels of fetuin-A, a well-described inhibitor of calcification, regulate the cell-dependent process of osteogenesis. The low circulating fetuin-A levels are associated with a greater prevalence and/or severity of Vascular calcification (VC) and increased risk for all-cause and cardiovascular mortality. However, high circulating fetuin-A levels appear to induce insulin resistance and, in non-dialyzed subjects with diabetic nephropathy, are directly related to VC burden. The emerging role of fetuin-A deficiency as a risk factor in dialysis patients was documented in cross-sectional studies demonstrating a significant correlation with all-cause and cardiovascular mortality. Additionally, Human fetuin-A is a negative acute phase protein involved in inflammatory diseases, thus being a potential physiological regulator of meprin activity. Fetuin-A is a broad-range protease inhibitor. Fetuin-A and cystatin C as endogenous proteolytic regulators of meprin activity broadens our understanding of the proteolytic network in plasma.
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分子量
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Calculated MW: 38.8 kDa
Observed MW: 55-60 kDa
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基因ID
- 197
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NCBI登录号
- NP_001613
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UniProt
- P02765
抗原
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