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AARS Protein (His tag)

This Recombinant AARS protein is expressed in Baculovirus infected Insect Cells.
产品编号 ABIN7320298
发货至: 中国
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Quick Overview for AARS Protein (His tag) (ABIN7320298)

抗原

See all AARS 蛋白
AARS (Alanyl-tRNA Synthetase (AARS))

蛋白类型

Recombinant

宿主

小鼠

资源

  • 1
  • 1
Baculovirus infected Insect Cells

纯度

> 88 % as determined by SDS-PAGE
  • 标记

    This AARS protein is labelled with His tag.

    原理

    Recombinant Mouse AARS/alanyl-tRNA synthetase Protein (His Tag)

    序列

    Met 1-Asn 968

    产品特性

    A DNA sequence encoding the mouse AARS (Q8BGQ7) (Met 1-Asn 968) was fused with a polyhistidine tag at the C-terminus.

    内毒素水平

    < 1.0 EU per μg of the protein as determined by the LAL method.
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  • 限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Please refer to the printed manual for detailed information.

    缓冲液

    Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
  • 抗原

    AARS (Alanyl-tRNA Synthetase (AARS))

    别名

    AARS/alanyl-tRNA synthetase

    背景

    Background: Alanyl-tRNA synthetase (AARS) belongs to the family of ligases, specifically those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis. Alanyl-tRNA synthetase (AlaRS) catalyzes synthesis of Ala-tRNA (Ala) and hydrolysis of mis-acylated Ser- and Gly-tRNA (Ala) at 2 different catalytic sites. Their role is not confined to catalyze the attachment of amino acids to transfer RNAs and thereby establish the rules of genetic code by virtue of matching the nucleotide triplet of anticodon with cognate amino acid. Under apoptotic conditions in cell culture, the full-length enzyme is secreted, and the two cytokine activities can be generated by leukocyte elastase, an extracellular protease. Secretion of this tRNA synthetase may contribute to apoptosis both by arresting translation and producing needed cytokines. This protein could be an attractive target of drugs against bacterial, fungal and parasitic infections. 

    Synonym: AI316495;C76919;sti

    分子量

    108.3 kDa

    UniProt

    Q8BGQ7
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