ALT Protein (His tag)
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Quick Overview for ALT Protein (His tag) (ABIN7194188)
抗原
See all ALT 蛋白蛋白类型
宿主
资源
纯度
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标记
- This ALT protein is labelled with His tag.
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原理
- Recombinant Rat GPT1/GPT Protein (His Tag)
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序列
- Met 1-Ser 496
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产品特性
- A DNA sequence encoding the rat Gpt1 (NP_112301.1) (Met 1-Ser 496) with a C-terminal polyhistidine tag was expressed.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Alanine Aminotransferase (ALT) (AA 1-496) protein (His-IF2DI Tag)
ALT 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Alanine Aminotransferase (ALT) (AA 1-496) protein (His-IF2DI Tag)ALT 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Alanine Aminotransferase (ALT) (AA 1-496) (Active) protein (His tag)ALT 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS, EAA Active
Alanine Aminotransferase (ALT) (AA 1-496) protein (His tag)ALT 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Alanine Aminotransferase (ALT) (AA 1-496) protein (GST tag)ALT 宿主: 人 宿主: 小麦胚 Recombinant AP, AA, ELISA, WB
Alanine Aminotransferase (ALT) protein (Myc-DYKDDDDK Tag)ALT 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 10 % glycerol, 0.5 mM TCEP
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- ALT (Alanine Aminotransferase (ALT))
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别名
- GPT1/GPT
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背景
- ALT1,Gpt,Gpt1,Alanine aminotransferase (ALT), also known as glutamate pyruvate transaminase (GPT), is a pyridoxal enzyme which belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family, Alanine aminotransferase subfamily. Gpt / Gpt1 / ALT catalyses the reversible interconversion of L-alanine and 2-oxoglutalate to pyruvate and L-glutamate, and plays a key role in the intermediary metabolism of glucose and amino acids. Gpt / Gpt1 / ALT is expressed in Liver, kidney, heart, and skeletal muscles and it expresses at moderate levels in the adipose tissue. As a key enzyme for gluconeogenesis, Gpt is a widely-used serum marker for liver injury. Two ALT isoenzymes have been identified, ALT1 and ALT2 (GPT1 and GPT2), which are encoded by separate genes and share significant sequence homology, but differ in their expression patterns. GPT1/GPT is widely distributed and mainly expressed in intestine, liver, fat tissues, colon, muscle, and heart, in the order of high to low expression level. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis.
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分子量
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Calculated MW: 55.0 kDa
Observed MW: 48 kDa
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NCBI登录号
- NP_112301
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UniProt
- A6HSC5
抗原
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